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Detection Limit of Minimal Residual Disease in Leukemia Using Fluorescence in situ Hybridization

Lee JH, Kim SY, Kim HH, Suh BK, Lee WB, Whang KT

  • KMID: 1967595
  • J Korean Pediatr Soc.
  • 2000 Oct;43(10):1364-1371.
PURPOSE: The close relationship between the size of a tumor burden and the curability of acute leukemia is well established. Therefore, it is very irnportant to detect residual leukemia accurately at low...
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Loop-mediated isothermal amplification assay for the detection of Salmonella spp. in pig feces

Kim YK, Kim HY, Jeon AB, Lee MH, Bae YC, Byun JW

  • KMID: 2307878
  • Korean J Vet Res.
  • 2014 Jun;54(2):113-115.
Salmonella are causative agents of gastroenteritis and systemic disease in animals. The invA gene was selected as a target sequence of loop-mediated isothermal amplification (LAMP) assay for diagnosis of Salmonella...
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Capillary Electrophoresis Detection of Hydrogen Peroxide by Using Titanium Ion and 4-(2-thiazolylazo)resorcinol

Phuong DV, Yoo H

A novel method for the detection of hydrogen peroxide in aqueous solution was developed via reaction between H₂O₂, trivalent titanium ion (Ti³⁺) and 4-(2-thiazolylazo) resorcinol (TAR), resulting in a ternary...
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The Comparsion of Polymerase Chain Reaction and Culture for Detection of Helicobacter pylori in Saliva

Paik IK, Han TH

  • KMID: 1889898
  • Korean J Lab Med.
  • 2003 Aug;23(4):258-262.
BACKGROUND: The presence of Helicobacter pylori DNA from saliva has been demonstrated by polymerase chain reaction; however, the culture of H. pylori is quite difficult. Because isolation of H. pylori...
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Detection Limit of Monoclonal B-Cells Using Multiplex PCR and Laser-Induced Fluorescence Capillary Electrophoresis

Lee SH, Moon Y, Song B, Lee HN, Lee A, Jung ES, Choi YJ, Lee KY, Kang CS, Park G

  • KMID: 1979060
  • Korean J Pathol.
  • 2011 Dec;45(6):582-588.
BACKGROUND: The identification of monoclonality has been widely used for making diagnoses of lymphoproliferative lesions. Awareness of the sensitivity and detection limit of the technique used would be important for...
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Evaluation of Two PCR-Hybridization Methods for the Detection of Mycobacterium tuberculosis

Kim JH, Jang SJ, Moon DS, Park YJ

  • KMID: 1866518
  • Korean J Lab Med.
  • 2003 Feb;23(1):32-38.
BACKGROUND: We evaluated two homemade polymerase chain reaction-hybridization (PCRH) assays for the detection of M. tuberculosis. METHODS: Two PCRH assays, using a digoxigenin-luminescent probe (DL-PCRH) and using a radioactive probe...
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Performance Evaluation of the QXDx BCR-ABL %IS Droplet Digital PCR Assay

Chung HJ, Hur M, Yoon S, Hwang K, Lim HS, Kim H, Moon HW, Yun YM

Accurate detection of BCR-ABL fusion transcripts at and below molecular response (MR) 4 (0.01% International Scale [IS]) is required for disease monitoring in patients with chronic myeloid leukemia (CML). We...
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Evaluation of the Real-Q RV Detection Kit for the Identification of Viruses That Result in Respiratory Infections

Lee E, Shin S, Kim M, Lee YK, Kang HJ, Kim HS, Kim JS, Song W, Kim HS

Viral respiratory infections are one of the most common infections worldwide. It is important to detect the virus early and precisely. In this study, we evaluated the limit of detection...
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The Evaluation of Immunochromatographic Assay kit for Rapid Detection of Hepatitis B Surface Antigen

Shin HS, Kim YB, Shin JW, Kim CK, Lee WS, Kim HK, Shin KS

  • KMID: 1541340
  • J Korean Soc Virol.
  • 1997 Dec;27(2):137-141.
We evaluated Immunochromatographic assay kit to screen HBsAg in human serum. When the reference HBsAg was applyed to ICA, HA and EIA kits, the limit of detection for HBsAg were...
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Risk Assessment on Carcinogenic Pesticides of Vegetables in Korea

Yoon JH, Lee JT, Chung Y, Shin D

  • KMID: 2282992
  • Korean J Prev Med.
  • 1999 Jun;32(2):155-161.
OBJECTIVES: The study was conducted to estimate the carcinogenic risks from the ingestion of some carcinogenic pesticides (CPs) in vegetables sampled at a local agricultural product market in Seoul. METHODS: After...
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Comparison of Detection of Escherichia coli O157 Between Culture After Acid Treatment and Polymerase Chain Reaction After Enrichment

Paik IK, Han TH

  • KMID: 2082773
  • Korean J Lab Med.
  • 2002 Oct;22(5):331-335.
BACKGROUND: The Polymerase Chain Reaction (PCR) for the Shiga toxin has been widely used for diagnosis of Shiga toxin-producing Escherichia coli (STEC) infection including Escherichia coli O157 (O157) instead of...
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Evaluation of Clinical Usefulness of PREG-Q(TM) as a Screening Test for Pregnancy

Kim CK, Lee WH, Lim JB, Won YC, Kwon OH

  • KMID: 1895345
  • J Lab Med Qual Assur.
  • 2004 Jun;26(1):207-210.
BACKGROUND: Among the many methods estimating the quantity of beta-hCG for pregnancy testing in urine, immunochromatography is one of most widely used semi-quantitative detection method for its convenience to use...
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Comparative Evaluation of the HPV28 Detection and HPV DNA Chip Test for Detecting and Genotyping Human Papillomaviruses

Shin E, Bae H, Song WK, Jung SK, Hwang YS

  • KMID: 2312229
  • Lab Med Online.
  • 2013 Oct;3(4):234-241.
BACKGROUND: The HPV28 Detection test (Seegene) is a real-time polymerase chain reaction assay that is designed for testing a total of 28 human papillomavirus (HPV) genotypes and estimating the approximate...
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Evaluation of Cobas Integra 700 and Distribution of High Sensitivity C-reactive Protein Levels in Koreans

Jang SS, Lee WC, Chun SI, Min WK

  • KMID: 2082771
  • Korean J Lab Med.
  • 2002 Oct;22(5):319-324.
BACKGROUND: Prospective studies suggest that C-reactive protein (CRP) is a novel and indepen-dent risk factor of coronary heart disease. High-sensitivity methods for CRP (hs-CRP) measurement are needed for this purpose....
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Performance Evaluation of the Elecsys Neuron-Specific Enolase Assay

Kim SK, Jeong TD, Lee W, Chun S, Min WK

BACKGROUND: Neuron-specific enolase (NSE) is an enzyme specifically found in neurons and neuroendocrine tissue. It is a common marker for small cell lung cancer diagnosis and is also useful as...
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Detection of Infectious Bursal Disease Virus by Double Antibody Sandwich ELISA

Jeon WJ, Chang BS, Park MJ, Lee EK, Joo HD, Kwon JH, Choi KS

Infectious bursal disease virus (IBDV) is responsible for a highly contagious disease of poultry causing severe immunosuppression in chickens. A double antibody sandwich ELISA (DAS-ELISA) was developed to detect IBDV...
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Strain-specific PCR Primers for the Detection of Prevotella intermedia ATCC 49046

Kim MJ, Min JB, Lim SA, Kook JK

  • KMID: 2170735
  • Int J Oral Biol.
  • 2011 Jun;36(2):79-82.
The aim of this study was to develop Prevotella intermedia ATCC 49046-specific PCR primers designed based on the nucleotide sequence of a DNA probe Pig28. The strain-specificity of the PCR...
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Performance Evaluation of LG AdvanSure HBV Real-Time QPCR Kit

Moon JJ, Lim CS, Lee CK, Oh HB

  • KMID: 2300006
  • Korean J Blood Transfus.
  • 2013 Aug;24(2):161-174.
BACKGROUND: Accurate quantitative testing of HBV DNA is very important for choosing antiviral treatment targets and evaluating treatment response in chronic HBV patients. We evaluated the performance of LG AdvanSure...
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Evaluation of Artus HBV LC PCR kit using SLAN Real-time PCR

Kim M, Lee DY, Chang YH, Lee JK, Hong SI, Hong YJ

  • KMID: 1466830
  • J Lab Med Qual Assur.
  • 2009 Dec;31(2):275-279.
BACKGROUND: Real-time PCR has been widely used not only for quantification?of disease-related genes but also for detection of bacteria or viruses. In this study, we evaluated the performance of Artus...
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Development of SCAR Markers for the Identification of Phytophthora katsurae Causing Chestnut Ink Disease in Korea

Lee DH, Lee SK, Lee SY, Lee JK

  • KMID: 2312693
  • Mycobiology.
  • 2013 Jun;41(2):86-93.
Sequence characterized amplified region (SCAR) markers are one of the most effective and accurate tools for microbial identification. In this study, we applied SCAR markers for the rapid and accurate...
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