- Marker Gene Transfer into Cells of Rat Achilles Tendon by In Vivo Electroporation
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Park JH, Park JW, Lee KS
- KMID: 2105746
- J Korean Orthop Assoc.
- 2001 Jun;36(3):221-226.
PURPOSE: To demonstrate and analyze quantitatively the expression of marker genes in the Achilles tendon of rats by direct DNA injection and electroporation. MATERIALS AND METHODS: Enhanced green fluorescent protein... -
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- Irreversible Electroporation: A Novel Image-Guided Cancer Therapy
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Lee EW, Thai S, Kee ST
- KMID: 2053440
- Gut Liver.
- 2010 Sep;4(Suppl 1):S99-S104.
Irreversible electroporation (IRE) is a novel tumor ablation technique using a non-thermal energy to create innumerable permanent nanopores in the cell membrane to disrupt cellular homeostasis. This disruption of cellular... -
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- Comparison of Various Transfection Methods in Human and Bovine Cultured Cells
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Jin L, Kim D, Roh S
- KMID: 2170785
- Int J Oral Biol.
- 2014 Dec;39(4):177-185.
- doi: 10.11620/IJOB.2014.39.4.177
Transfection is a gene delivery tool that is a popular means of manipulating cellular properties, such as induced pluripotent stem cell (iPSC) generation by reprogramming factors (Yamanaka factors). However, the... -
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- RE: Irreversible Electroporation of a Hepatocellular Carcinoma Lesion Adjacent to a Transjugular Intrahepatic Portosystemic Shunt Stent Graft
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Pua U
- KMID: 1711498
- Korean J Radiol.
- 2014 Feb;15(1):181-182.
- doi: 10.3348/kjr.2014.15.1.181
No abstract available. -
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- Optimizing in vivo gene transfer into mouse corpus cavernosum by use of surface electroporation
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Song KM, Choi MJ, Kwon MH, Ghatak K, Park SH, Ryu DS, Ryu JK, Suh JK
- KMID: 2133287
- Korean J Urol.
- 2015 Mar;56(3):197-204.
- doi: 10.4111/kju.2015.56.3.197
PURPOSE: Electroporation is known to enhance the efficiency of gene transfer through a transient increase in cell membrane permeability. The aim of this study was to determine the optimal conditions... -
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- Irreversible Electroporation of a Hepatocellular Carcinoma Lesion Adjacent to a Transjugular Intrahepatic Portosystemic Shunt Stent Graft
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Niessen C, Jung EM, Wohlgemuth WA, Trabold B, Haimerl M, Schreyer A, Stroszczynski C, Wiggermann P
- KMID: 1711436
- Korean J Radiol.
- 2013 Oct;14(5):797-800.
- doi: 10.3348/kjr.2013.14.5.797
We report in a 65-year-old man hepatocellular carcinoma adjacent to a transjugular intrahepatic portosystemic shunt stent-graft which was successfully treated with irreversible electroporation (IRE). IRE is a new non-thermal tissue... -
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- Irreversible Electroporation in Patients with Pancreatic Cancer :Angel and Devil
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Keum J, Lee HS, Kang H, Jo JH, Chung MJ, Park JY, Park SW, Song SY, Bang S
- KMID: 2455392
- J Dig Cancer Res.
- 2019 Jun;7(1):26-30.
- doi: 10.0000/jdcr.2019.7.1.26
Pancreatic cancer has a very poor prognosis. Complete surgical resection remains the only current curative treatment. Locally advanced pancreatic cancer (LAPC) is considered as unresectable because of involvement of celiac... -
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- Effect of IP3 and ryanodine treatments on the development of bovine parthenogenetic and reconstructed embryos
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Ahn GJ, Lee BC, Hwang WS
- KMID: 1103521
- J Vet Sci.
- 2001 Aug;2(2):131-137.
For parthenogenetic activation as a model system of nuclear transfer, microinjection and electroporation as activation treatments in bovine metaphase II oocytes were administered to each of three groups as follows:... -
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- Isolation and Characterization of Chicken NPAS3
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Shin J, Jeong HY, Lee KE, Kim J
- KMID: 1460702
- Exp Neurobiol.
- 2010 Sep;19(2):71-74.
- doi: 10.5607/en.2010.19.2.71
Here we describe characterization of chicken neuronal Per-Arnt-Sim domain 3 (NPAS3) gene during embryogenesis including examinations of expression pattern and function of the gene. RTPCR assay showed that the primary... -
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- Irreversible electroporation for the treatment of pancreatic neuroendocrine tumors
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Papamichail M, Ali A, Pizanias M, Peddu P, Karani J, Heaton N
- KMID: 2351298
- Korean J Hepatobiliary Pancreat Surg.
- 2016 Aug;20(3):116-120.
- doi: 10.14701/kjhbps.2016.20.3.116
BACKGROUNDS/AIMS: Resection or enucleation is currently the treatment of choice for small pancreatic neuroendocrine tumors (NETs). Irreversible electroporation is a novel ablative method that is used for locally advanced pancreatic... -
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- Highly Efficient Electroporation-mediated Transformation into Edible Mushroom Flammulina velutipes
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Kim JK, Park YJ, Kong WS, Kang HW
- KMID: 2312600
- Mycobiology.
- 2010 Dec;38(4):331-335.
In this study, we developed an efficient electroporation-mediated transformation system featuring Flammulina velutipes. The flammutoxin (ftx) gene of F. velutipes was isolated by reverse transcription-PCR. pFTXHg plasmid was constructed using... -
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- DNA vaccines targeting human papillomavirus-associated diseases: progresses in animal and clinical studies
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Han KT, Sin JI
- KMID: 2167720
- Clin Exp Vaccine Res.
- 2013 Jul;2(2):106-114.
- doi: 10.7774/cevr.2013.2.2.106
Human papillomavirus (HPV) infection is a major cause of cervical cancer and its precancerous diseases. Cervical cancer is the second deadliest cancer killer among women worldwide. Moreover, HPV is also... -
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- Improvement of Transformation System in Filamentous Fungus Aspergillus oryzae
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Lee JW, Yun CD, Hahm YT
- KMID: 2243203
- Korean J Med Mycol.
- 2001 Mar;6(1):1-8.
Aspergillus oryzae is a filamentous fungus classified in the group Aspergillaceae Ascomycetes. A. oryzae is an important microorganism for industrial production of enzymes and fermented food products. It secrets large... -
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- Optimal salt concentration of vehicle for plasmid DNA enhances gene transfer mediated by electroporation
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Lee MJ, Cho SS, Jang HS, Lim YS, You JR, Park JW, Suh HR, Kim JA, Park JS, Kim DK
- KMID: 931876
- Exp Mol Med.
- 2002 Sep;34(4):265-272.
In vivo electroporation has emerged as a leading technology for developing nonviral gene therapies, and the various technical parameters governing electroporation efficiency have been optimized by both theoretical and... -
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- Factors affecting transformation efficiency of BCG with a Mycobacterium-Escherichia coli shuttle vector pYUB18 by electroporation
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Cho SN, Hwang JH, Park S, Chong Y, Kim SK, Song CY, Kim JD
- KMID: 711538
- Yonsei Med J.
- 1998 Apr;39(2):141-147.
- doi: 10.3349/ymj.1998.39.2.141
BCG has been one of the vehicles for multi-recombinant vaccine. However, low transformation efficiency of BCG with plasmid DNA hampered studies involving expression of foreign antigens in BCG. In an... -
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- Comparison of Ectopic Gene Expression Methods in Rat Neural Stem Cells
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Kim W, Kim JH, Kong SY, Park MH, Sohn UD, Kim HJ
- KMID: 1432759
- Korean J Physiol Pharmacol.
- 2013 Feb;17(1):23-30.
- doi: 10.4196/kjpp.2013.17.1.23
Neural stem cells (NSCs) have the ability to proliferate and differentiate into various types of cells that compose the nervous system. To study functions of genes in stem cell biology,... -
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- A Simple, Quantitative Method for Assessing Angiogenic Genes Using Skeletal Muscle by Electroporation-Mediated Naked DNA Delivery
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Kim HJ, Jang HS, Kim JM, Lee YS, Kim KR, Kim JA, Jeon ES, Byun JH, Kim DK
- KMID: 2093869
- Korean Circ J.
- 2003 Apr;33(4):321-332.
- doi: 10.4070/kcj.2003.33.4.321
BACKGROUND AND OBJECTIVES: For the development of an arteriogenic gene therapy in peripheral artery occlusive disease, we developed a novel angiogenesis assay, with electroporation-mediated naked DNA delivery to the skeletal... -
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- Increased in vivo immunological potency of HB-110, a novel therapeutic HBV DNA vaccine, by electroporation
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Kim CY, Kang ES, Kim SB, Kim HE, Choi JH, Lee DS, Im SJ, Yang SH, Sung YC, Kim BM, Kim BG
- KMID: 1072947
- Exp Mol Med.
- 2008 Dec;40(6):669-676.
- doi: 10.3858/emm.2008.40.6.669
Pulse-induced permeabilization of cellular membranes, generally referred to as electroporation (EP), has been used for years as a tool to increase macromolecule uptake in tissues, including nucleic acids, for gene... -
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- Pathology of non-thermal irreversible electroporation (N-TIRE)-induced ablation of the canine brain
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Rossmeisl , Garcia PA, Roberston , Ellis TL, Davalos RV
- KMID: 1712310
- J Vet Sci.
- 2013 Dec;14(4):433-440.
- doi: 10.4142/jvs.2013.14.4.433
This study describes the neuropathologic features of normal canine brain ablated with non-thermal irreversible electroporation (N-TIRE). The parietal cerebral cortices of four dogs were treated with N-TIRE using a dose-escalation... -
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- Improvement of DNA vaccination by adjuvants and sophisticated delivery devices: vaccine-platforms for the battle against infectious diseases
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Grunwald T, Ulbert S
- KMID: 2049102
- Clin Exp Vaccine Res.
- 2015 Jan;4(1):1-10.
- doi: 10.7774/cevr.2015.4.1.1
Advantages of DNA vaccination against infectious diseases over more classical immunization methods include the possibilities for rapid manufacture, fast adaptation to newly emerging pathogens and high stability at ambient temperatures.... -
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