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Codon optimization of the rabbit hemorrhagic disease virus (RHDV) capsid gene leads to increased gene expression in Spodoptera frugiperda 9 (Sf9) cells

Gao J, Meng C, Chen Z, Li C, Liu G

Rabbit hemorrhagic disease (RHD) is contagious and highly lethal. Commercial vaccines against RHD are produced from the livers of experimentally infected rabbits. Although several groups have reported that recombinant subunit...
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Simple Purification of Adeno-Associated Virus-DJ for Liver-Specific Gene Expression

Liu J, Moon YA

Recombinant gene expression using adeno-associated viruses (AAVs) has become a valuable tool in animal studies, as they mediate safe expression of transduced genes for several months. The liver is a...
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Development of a novel enzyme-linked immunosorbent assay to detect anti-IgG against swine hepatitis E virus

Lee WJ, Shin MK, Cha SB, Yoo HS

Swine hepatitis E virus (HEV) is widespread throughout pigs in both developing and industrialized countries. This virus is an important zoonotic agent and a public concern worldwide. Infected pigs are...
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Enhancing mucosal immunity in mice by recombinant adenovirus expressing major epitopes of porcine circovirus-2 capsid protein delivered with cytosine-phosphate-guanosine oligodeoxynucleotides

Chang HT, He XY, Liu YF, Chen L, Guo QH, Yu QY, Zhao J, Wang XW, Yang X, Wang CQ

A recombinant replication-defective adenovirus expressing the major epitopes of porcine circovirus-2 (PCV-2) capsid protein (rAd/Cap/518) was previously constructed and shown to induce mucosal immunity in mice following intranasal delivery. In...
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Comparative study of codon substitution patterns in foot-and-mouth disease virus (serotype O)

Ahn I, Bae SE, Son HS

We compared genetic variations in the VP1 gene of foot-and-mouth disease viruses (FMDVs) isolated since 2000 from various region of the world. We analyzed relative synonymous codon usage (RSCU) and...
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