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The Combination of Real-Time PCR and HPLC for the Identification of Non-Tuberculous Mycobacteria

Park JS, Choi JI, Lim JH, Ahn JJ, Jegal Y, Seo KW, Ra SW, Jeon JB, Lee SH, Kim SR, Jeong J

We used HPLC and AdvanSure real-time PCR (LG Life Sciences, Korea) to retrospectively analyze non-tuberculous mycobacteria (NTM) in 133 clinical specimens. The specimens were culture-positive for NTM and the HPLC...
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Pseudo-outbreak of Stenotrophomonas maltophilia Due to Contamination of Bronchoscope

Ahn GY, Yu FN, Jang SJ, Kim DM, Park G, Moon DS, Park YJ

BACKGROUND: We noticed an abrupt increase in the isolation of Stenotrophomonas maltophilia from bronchoalveolar lavage (BAL) specimens collected at Chosun University Hospital. We performed surveillance cultures in order to...
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Assessment of the Quantitative Ability of AdvanSure TB/NTM Real-Time PCR in Respiratory Specimens by Comparison with Phenotypic Methods

Lee H, Park KG, Lee G, Park J, Park YG, Park YJ

Accurate quantification of mycobacterial load is important to evaluate disease severity and to monitor the course of treatment in tuberculosis (TB). We evaluated the quantitative capability of the AdvanSure TB/NTM...
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Computed Tomography Findings of Community-Acquired Stenotrophomonas Maltophilia Pneumonia in an Immunocompetent Patient: A Case Report

Cha YK, Kim JS, Park SY, Oh JY, Kwon JH

Stenotrophomonas maltophilia (S. maltophilia) is a rare, but globally emerging gram-negative multiple-drug-resistant organism usually found in a nosocomial setting in immunocompromised patients. To our best knowledge, computed tomography (CT) features...
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Usefulness of Quantitative Endotracheal Aspirate Cultures in Intensive Care Unit Patients with Suspected Pneumonia

Shin YM, Oh YM, Kim MN, Shim TS, Lim CM, Lee SD, Koh Y, Kim WS, Kim DS, Hong SB

It is difficult to differentiate pathogens responsible for pneumonia or colonization in patients with an endotracheal tube or in patients that have undergone tracheostomy. We evaluated the clinical usefulness of...
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Evaluation of Peptide Nucleic Acid Probe-based Real-time PCR for Detection of Mycobacterium tuberculosis Complex and Nontuberculous Mycobacteria in Respiratory Specimens

Choi YJ, Kim HJ, Shin HB, Nam HS, Lee SH, Park JS, Park KS, Baek KA

BACKGROUND: A peptide nucleic acid (PNA) probe-based real-time PCR (PNAqPCR(TM) TB/NTM detection kit; PANAGENE, Korea) assay has been recently developed for the simultaneous detection of Mycobacterium tuberculosis complex (MTBC) and...
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Recent Trends in Clinically Significant Nontuberculous Mycobacteria Isolates at a Korean General Hospital

Kim HS, Lee Y, Lee S, Kim YA, Sun YK

Lung disease caused by nontuberculous mycobacteria (NTM) represents an increasing proportion of all mycobacterial diseases. We investigated recent occurrences of NTM and evaluated the clinical significance of NTM isolates from...
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