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Rapid detection of Theileria sergenti by polymerase chain reaction

Choi EJ, Kang SW, Kweon CH, Jeong WS, Yoon YD, Song HJ

Four separate pairs of oligonucleotide primers within the coding region in a T. sergenti 33-kDa surface protein gene were selected to detect T. sergenti by PCR. The specificity of PCR-amplified...
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Efficacy of a DNA Vaccine Carrying Eimeria maxima Gam56 Antigen Gene against Coccidiosis in Chickens

Xu J, Zhang Y, Tao J

To control coccidiosis without using prophylactic medications, a DNA vaccine targeting the gametophyte antigen Gam56 from Eimeria maxima in chickens was constructed, and the immunogenicity and protective effects were evaluated....
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A Novel Recombinant BCG Vaccine Encoding Eimeria tenella Rhomboid and Chicken IL-2 Induces Protective Immunity Against Coccidiosis

Wang Q, Chen L, Li J, Zheng J, Cai N, Gong P, Li S, Li H, Zhang X

  • KMID: 1792178
  • Korean J Parasitol.
  • 2014 Jun;52(3):251-256.
A novel recombinant Bacille Calmette-Guerin (rBCG) vaccine co-expressed Eimeria tenella rhomboid and cytokine chicken IL-2 (chIL-2) was constructed, and its efficacy against E. tenella challenge was observed. The rhomboid gene...
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Plasmodium falciparum Genotype Diversity in Artemisinin Derivatives Treatment Failure Patients along the Thai-Myanmar Border

Congpuong , Hoonchaiyapoom T, Inorn K

Genetic characteristics of Plasmodium falciparum may play a role in the treatment outcome of malaria infection. We have studied the association between diversity at the merozoite surface protein-1 (msp-1), msp-2,...
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A Rapid Diagnostic Test for Toxoplasmosis using Recombinant Antigenic N-terminal Half of SAG1 Linked with Intrinsically Unstructured Domain of GRA2 Protein

Song KJ, Yang Z, Chong CK, Kim JS, Lee KC, Kim TS, Nam HW

  • KMID: 1792140
  • Korean J Parasitol.
  • 2013 Oct;51(5):503-510.
Toxoplasma gondii is an apicomplexan parasite with a broad host range of most warm-blooded mammals including humans, of which one-thirds of the human population has been infected worldwide which can...
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Genotype of Toxoplasma gondii from Blood of Stray Cats in Gyeonggi-do, Korea

Kim HY, Kim YA, Lee HS, Rhie HG, Cho SH, Yu JR, Lee SE

Genotyping of Toxoplasma gondii has been performed in 23 PCR positive blood samples from stray cats in Korea. We used 2 separate PCR-restriction fragment length polymorphism (RFLP) patterns of SAG2...
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Identification of Antigenic Proteins in Trichomonas vaginalis

Lee HY, Hyung S, Lee JW, Kim J, Shin MH, Ryu JS, Park SJ

Trichomoniasis is a sexually transmitted disease due to infection with Trichomonas vaginalis, and it can cause serious consequences for women's health. To study the virulence factors of this pathogen, T....
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ELISA detection of vivax malaria with recombinant multiple stage-specific antigens and its application to survey of residents in endemic areas

Kim S, Ahn HJ, Kim TS, Nam HW

An ELISA was developed for the diagnosis of vivax malaria using multiple stage-specific recombinant antigens of Plasmodium vivax. The DNA from the whole blood of a malaria patient was used...
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Analysis of antigenic domain of GST fused major surface protein (p30) fragments of Toxoplasma gondii

Nam HW, Im KS, Baek EJ, Choi WY, Cho SY

Antigenic domain of major surface protein (p30) of Toxoplasma gondii was analyzed after polymerase chain reaction (PCR) of its gene fragments. Hydrophilic or hydrophobic moiety of amino acid sequences were...
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Decreasing effect of an anti-Nfa1 polyclonal antibody on the in vitro cytotoxicity of pathogenic Naegleria fowleri

Jeong SR, Kang SY, Lee SC, Song KJ, Im KI, Shin HJ

The nfa1 gene was cloned from a cDNA library of pathogenic Naegleria fowleri by immunoscreening; it consisted of 360 bp and produced a 13.1 kDa recombinant protein (rNfa1) that showed...
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Vaccination against Murine Toxoplasmosis Using Recombinant Toxoplasma gondii SAG3 Antigen Alone or in Combination with Quil A

Lee YH, Shin DW, lee JH, Nam HW, Ahn MH

PURPOSE: Surface antigen 3 (SAG3) of Toxoplasma gondii is very similar in structure to the major surface antigen 1 (SAG1). Although numerous studies have supported the importance of SAG1 in...
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