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The production and characterization of anti-Naegleria fowleri monoclonal antibodies

Ryu JS, Im KI

Naegleria fowleri, a free-living amoeba commonly found in moist soil and fresh water, enters the body via the nasal mucosa and migrates along the olfactory nerve to the brain, where...
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The protective effects of monoclonal antibodies in mice from Naegleria fowleri infection

Soh EY, Shin HJ, Im K

Protective effects of monoclonal antibodies against N. fowleri were comparatively studied. BALB/c mice were treated with two types of monoclonal antibodies, Nf 2 and Nf 154, before and after the...
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Passive transfer of immunity against Cryptosporidium infection in neonatal mice using monoclonal antibodies

Cho MH

Monoclonal antibodies (mAb) against merozoites and sporozoites of the protozoan parasite Cryptosporidium parvum were examined for potential modulation of cryptosporidial infections in vivo by daily oral mAb administration to oocyst-inoculated...
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Immunohistochemical observation on the antigens inducing IgG and IgM antibodies against sparganum

Kim CH, Choi WS

Localization and characterization of the antigenic components of sparganum which induced IgG and IgM antibodies in the host were studied by immunohistochemical techniques and SDS-PAGE and Western blotting. The antigen...
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Serodiagnosis of cysticercosis by ELISA-inhibition test using monoclonal antibodies

Yong TS, Yeo IS, Seo JH, Chang JK, Lee JS, Kim TS, Jeong GH

Monoclonal antibodies (Mabs) were produced against crude scolex extract of T. solium metacestodes, and applied to ELISA-inhibition test for improving the specificity of serodiagnosis of human cysticercosis. Four hybridomas...
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Serodiagnosis of human paragonimiasis by ELISA-inhibition test using monoclonal antibodies

Yong TS, Seo JH, Yeo IS

ELISA-inhibition test using Paragonimus westermani specific monoclonal antibody (Mab) was investigated to improve the diagnostic specificity of paragonimiasis. By cell fusion, one hybridoma clone secreting anti-P. westermani specific Mab was...
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Diagnosis and epidemiology of toxoplasmosis in Korea

Choi WY

Toxoplasma gondii can cause congenital as well as postnatal toxoplasmosis in humans and animals, and thereby elicit various kinds and grades of clinical manifestations. In the human host, especially the...
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Component proteins in crude extract of adult Paragonimus westermani purified by immunoaffinity chromatography using monoclonal antibodies

Kang SY, Kong Y, Cho SY

The nature of 2 component proteins in crude saline extract of adult Paragonimus westermani was investigated. By immunoaffinity chromatography using monoclonal antibodies (MAb) as ligands, the proteins were purified from...
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Detection of IgG and IgM antibodies with ELISA technique in human trichomoniasis

Yi MR, Shin MH, Leem MH, Ryu JS, Ahn MH, Min DY

The direct wet mount examination of vaginal secretion, widely applied for the diagnosis of Trichomonas vaginalis infection in woman patients, is rapid and economical, however, the sensitivity of this technique...
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Detection and characterization of excretory/secretory proteins from Toxoplasma gondii by monoclonal antibodies

Son ES, Nam HW

Excretory/secretory proteins (ESP) from Toxoplasma gondii were analyzed to define the function in the penetration process into host cells. Whole ESP obtained at 37 degrees C were composed of 15...
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Toxoplasma gondii: ultrastructural localization of specific antigens and inhibition of intracellular multiplication by monoclonal antibodies

Lee BY, Ahn MH, Kim HC, Min DY

This experiment was focused on the characterization of anti-Toxoplasma monoclonal antibodies (mAbs) and the effect of mAbs on the parasite invasion of mouse peritoneal macrophages. Twenty eight mAbs including...
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Detection of antibodies in serum and cerebrospinal fluid to Toxoplasma gondii by indirect latex agglutination test and enzyme-linked immunosorbent assay

Choi WY, Nam HW, Youn JH, Kim DJ, Kong Y, Kang SY, Cho SY

Sensitivity of anti-Toxoplasma antibody (IgG) test by enzyme-linked immunosorbent assay (ELISA) was evaluated in comparison with indirect latex agglutination (ILA) using 2,016 paired human samples of serum and cerebrospinal fluid...
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Immunohistochemical study on the antigenicity of each organ structure of Clonorchis sinensis

Kim J, Chai JY, Kho WG, Cho KH, Lee SH

An immunohistochemical study was performed to demonstrate comparative antigenicity of each body structure of the liver fluke, Clonorchis sinensis, such as the digestive tract, reproductive organs, excretory system, tegument and...
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Applicability of ABC-ELISA and protein A-ELISA in serological diagnosis of cysticercosis

Lee JH, Kong Y, Ryu JY, Cho SY

Specific antibody test in serum and cerebrospinal fluid (CSF) is still the main mode of serological diagnosis of cysticercosis. Of different techniques of antibody test, enzyme-linked immunosorbent assay (micro-ELISA) has...
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IgA response in mice infected with Neodiplostomum seoulensis

Huh S, Lee SU, Won MH, Jeong YG, Kwon YH, Choi CS

To observe the production of IgA in Balb/c mice with neodiplostomiasis, 20 mice were infected with each 200 metacercariae of Neodiplostomum seoulenis. Sera and the duodenums were obtained 3, 7,...
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Serum IgE levels in rats infected with Paragonimus westermani

Shin MH, Ryu JS, Min DY

Paragonimus westermani is a common fluke in Korea. The present study aimed to determine serum total IgE and specific IgG levels in experimental paragonimiasis of rats. Each Wistar rat was...
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Immune reactions between excretory-secretory antigens and specific antibodies of Clonorchis sinensis before and after praziquantel treatment in experimentally infected rabbits

Kim SI

This study was designed to evaluate the humoral immune reactions in clonorchiasis before and after praziquantel treatment. Rabbits were infected with 150 or 450 metacercariae, treated on 4 and 8...
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The effects of antibodies and complement in macrophage-mediated cytotoxicity on metacercariae of the lung fluke, Paragonimus westermani

Min DY, Ahn MH, Kim KM, Leem MH, Park SY

Paragonimus westermani is a tissue migrating parasite in the early stage until arriving at lung, and most of the parasites spend their life spans there. Considerable immune responses including activation...
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Immunohistochemical study on the antigenicity of body compartments of Paragonimus westermani

Lee SH, Sung SH, Chai JY

Production of circulating specific antibodies to the lung fluke (Paragonimus westermani) by its host is well known and used in various kinds of immunodiagnostic methods. However, it has not been...
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Changes in blastogenic responses and antibody titers of mice infected with Toxoplasma gondii

Shin DW, Lee YH, Na YE, Kwon DS

This study was performed to observe the cell-mediated and humoral immune responses in mice which were infected with Beverley, Fukaya and ME49 strain of Toxoplasma gondii, respectively. The blastogenic responses...
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