Korean J Dermatol.
1977 Mar;15(1):27-30.
New Plexiglass Hanging Drop Culture Apparatus
Abstract
- The purpose of this paper is to describe a new apparatus which utilizes the principle of Van Tieghems hanging drop culture method. After using various methods of culturing fungi authors have found van Tieghems method to yield the most satifsymg result. Until now, this method has been slightly more tedious due to durability, storage, and quantity of culture necessary for each species. The new apparatus consists of 20mm plexiglass tubing cut in 16mm lengths and attache to a 16cm x12cm x2mm plexiglass plate with paraffin or manicure. These tubes a.re ranged in rows approximately 6cm apart with at least 8mm between tubes. Plates of varying sizes can be made to accornodate specific experimente. The authors developed thse inexpensive unbreakable plexiglass plates to allow for aimultaneous growth of various fungi and therefore more accurate comparison. Since these plates can be stored and handled easily, most af the disadvantages of using van Tieghems hanging drop culture rnethod have been eliminated.
