Korean J Ophthalmol.  2010 Jun;24(3):163-168. 10.3341/kjo.2010.24.3.163.

The Anti-angiogenic Effect of Chlorogenic Acid on Choroidal Neovascularization

• Kim C
• Yu HG
• Sohn J

• KMID: 945987
• DOI: http://doi.org/10.3341/kjo.2010.24.3.163

Abstract

PURPOSE
To evaluate the inhibitory effect of chlorogenic acid on laser-induced choroidal neovascularization (CNV) in a rat model. METHODS: Intraperitoneal injection of chlorogenic acid (10 mg/kg) was inititated one day prior to laser photocoagulation and continued for eight days. Eyes were removed 14 days after laser photocoagulation. Fluorescein angiography was employed at seven and 14 days to assess the CNV lesions, and histological examination was performed. Quantification of CNV size and leakage were performed both in histological sections and fluorescein angiography in order to compare the inhibitory effects of chlorogenic acid on CNV with the results of the control. RESULTS: Histological analysis showed no significant difference in CNV size between the treated and control groups. However, CNV leakage on fluorescein angiography had significantly decreased in the chlorogenic acid-treated group at 14 days after laser photocoagulation compared with that of the control group. In addition, CNV size on fluorescein angiography had significantly decreased in the treated group at seven and 14 days. CONCLUSIONS: These results suggest that chlorogenic acid has anti-angiogenic effects on CNV and may be useful as an inhibitor in the treatment or prevention of neovascular age-related macular degeneration.

Keyword

Angiogenesis inhibitors; Antioxidants; Chlorogenic acid; Choroidal neovasculaization

MeSH Terms

Angiogenesis Inhibitors/*administration & dosage
Animals
Capillary Permeability/drug effects
Chlorogenic Acid/*administration & dosage
Choroid/pathology
Choroidal Neovascularization/diagnosis/etiology/*physiopathology
Fluorescein Angiography
Injections, Intraperitoneal
Laser Coagulation
Radiation Injuries
Rats
Rats, Inbred BN

Figure

• 

  Fig. 1 Hematoxylin-eosin stainings of representative areas of choroidal neovascularization (CNV) at the sites of laser-induced trauma in the control group (A) and chlorogenic acid-treated group (B). Intraperitoneal injection of chlorogenic acid (10 mg/kg) was started one day prior to laser photocoagulation and continued for eight days. The eyes were removed 14 days after laser photocoagulation. Note the presence of laser-induced CNV in each group (between white arrows).

• 

  Fig. 2 (A) In order to evaluate changes in the choroidal neovascularization lesion, the ratios of B (the thickness from the bottom of the pigmented choroidal layer to the top of the neovascular membrane) to C (the thickness of the intact-pigmented choroid adjacent to the lesion) were compared. (B) There was no significant difference in the B/C ratio between the chlorogenic acid-treated and control groups.

• 

  Fig. 3 (A) Representative histological image for the quantification of choroidal neovascularization (CNV) size in a sagittal section using a computer-assisted image analysis system. (B) No significant difference in the mean CNV area was observed between chlorogenic acid-treated and control groups.

• 

  Fig. 4 Angiographic analysis of choroidal neovascularization (CNV) leakage sevenand 14 days after laser photocoagulation between chlorogenic acid-treated and control groups. (A) Chlorogenic acid-treated rats developed reduced CNV and leakage at 14 days compared with those of control rats. (B) Histogram of angiographic leakage grades. Pathologically significant leakage (grade 3) was observed in 6.5% of CNV lesions in chlorogenic acid-treated rats at 14 days, while it was observedin a significantly higher proportion (61.8%) of lesions in control rats (p<0.001). FAG=fluorescein angiography.

• 

  Fig. 5 Angiographic analysis of choroidal neovascularization (CNV) size demonstrated that the chlorogenic acid-treated group had a significant decrease in CNV area at day 7 and day 14 compared with those of the control group. FAG=fluorescein angiography.

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