Korean J Gastroenterol.  2007 Feb;49(2):72-78.

Expression of Peroxisome Proliferator-activated Receptor (PPAR)gamma in Helicobacter pylori-infected Gastric Epithelium

• Son SH
• Kim HK
• Ji JS
• Cho YS
• Kim SS
• Chae HS
• Choi MG
• Han SW
• Choi KY
• Chung IS
• Shin OR

Abstract

BACKGROUND/AIMS
Peroxisome proliferator-activated receptorgamma (PPARgamma), a nuclear transcription factor, plays a critical role in the regulation of gene expression associated with inflammation and cancer. PPARgamma is expressed in human gastric cancer as well as in colon cancer. Activation of PPARgamma by ligand produces pro-apoptotic effect and ameliorate growing of cancer cells. Helicobacter pylori (H. pylori) is a main etiologic agent for gastric inflammation, and raises cell turnover in gastric epithelium. Longstanding infection with this organism is related with the development of non-cardiac gastric cancer. The aim of this study was to investigate the effect of H. pylori on the expression of PPARgamma protein and mRNA in chronic gastritis.
METHODS
Gastric biopsy samples were taken from H. pylori infected (n=18) and non-infected (n=21) patients during endoscopic examination. PPARgamma expressions were assessed by real time polymerase chain reaction and immunohistochemistry.
RESULTS
PPARgamma was localized to the nuclei of the foveolar epithelial cells in both infected and non-infected mucosa. PPARgamma protein expression was higher in H. pylori infected patients than in non-infected patients (3.8+/-0.4 vs. 2.6+/-1.0, H. pylori infected and non-infected, respectively; p<0.05). However, PPARgamma mRNA levels were not significantly different between the two groups (24+/-18 vs. 29+/-25, H. pylori infected and noninfected, respectively).
CONCLUSIONS
PPARgamma expression is increased in the gastric mucosa of H. pylori infected chronic gastritis, which suggests a certain role of PPARgamma in the mucosal inflammatory reaction to H. pylori infection.

Keyword

Helicobacter pylori; Peroxisome proliferator-activated receptor gamma

MeSH Terms

Adult
Colonic Neoplasms/metabolism/microbiology/pathology
Computer Systems
Female
Gastric Mucosa/*metabolism/microbiology/pathology
Gastritis/*metabolism/microbiology/pathology
Helicobacter Infections/*metabolism/microbiology
*Helicobacter pylori
Humans
Immunohistochemistry
Male
Middle Aged
PPAR gamma/*metabolism
Reverse Transcriptase Polymerase Chain Reaction
Stomach Neoplasms/metabolism/microbiology/pathology