Korean J Lab Med.  2008 Aug;28(4):299-306. 10.3343/kjlm.2008.28.4.299.

Evaluation of Combination of BACTEC Mycobacteria Growth Indicator Tube 960 System and Ogawa Media for Mycobacterial Culture

• Bae E
• Im JH
• Kim SW
• Yoon NS
• Sung H
• Kim MN
• Shim TS

• KMID: 854893
• DOI: http://doi.org/10.3343/kjlm.2008.28.4.299

Abstract

BACKGROUND: The combined use of liquid media and solid media is recommended for mycobacterial culture. We evaluated diagnostic performance of combination of BACTEC Mycobacteria Growth Indicator Tube (MGIT; Becton Dickinson, USA) and 2% Ogawa media (Korean Institute of Tuberculosis, Korea) for recovery of mycobacteria. METHODS: In September 2007, 1,764 specimens from 1,059 patients were cultured with MGIT and Ogawa. Acid fast bacilli (AFB) smear was fluorochrome-stained. The isolates were identified into Mycobacterium tuberculosis (MTB) and nontuberculous mycobacteria (NTM) with PCR using Seeplex TB Detection Kit (Seegene, Korea). Recovery rate, time to detection (TTD), contamination rate, mixed growth rate and species distribution were analyzed. RESULTS: Two hundred thirty-five specimens (13.3%) from 165 patients (15.6%) were positive for mycobacterial culture. Recovery rates of mycobacteria from the group using both media, MGIT only, and Ogawa only were 13.3%, 12.1%, and 7.8%, respectively. While MGIT recovered 98.9% of MTB and 79.7% of NTM, Ogawa recovered 65.9% of MTB and 54.1% of NTM. TTDs of total mycobacteria/MTB/NTM in MGIT and Ogawa were 10.6/11.4/9.7 days and 31/29/33 days, respectively. MGIT TTDs of total mycobacteria/MTB/NTM from AFB-positive specimens were significantly shorter than those of AFB-negative specimens; 8.2/9.5/4.4 days vs 11.6/12.7/10.7 days. Contamination and mixed growth rate of MGIT were 9.6% and 3.7%. Primary culture of Ogawa recovered 1 MTB and 1 NTM among the 170 MGIT-contaminated specimens and 38 mycobacteria among 66 specimens that showed mixed cultures of MGIT. CONCLUSIONS: MGIT warrants sensitive and rapid isolation of mycobacteria. However, the combination of MGIT and Ogawa is more desirable to recover mycobacteria in the case of contaminations or mixed cultures.

Keyword

BACTEC MGIT 960 system; Ogawa media; Mycobacterium tuberculosis; Nontuberculous mycobacteria; AFB smear

MeSH Terms

*Culture Media
False Positive Reactions
Humans
Mycobacterium/*growth & development/isolation & purification
Mycobacterium Infections/*diagnosis/microbiology
Mycobacterium tuberculosis/*growth & development/isolation & purification
Reagent Kits, Diagnostic
Sensitivity and Specificity
Sputum/microbiology
Time Factors

Figure

• 

  Fig. 1. Processing scheme of mycobacterial culture in this study.

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