Korean J Clin Pathol.  2000 Aug;20(4):384-391.

Detection of Mycobacterium tuberculosis using BACTEC Mycobacteria Growth Indicator Tube(MGIT) 960 system: Comparison with BACTEC 460 TB system and Ogawa Media

• Yi JY
• Kim JP
• Shin JH
• Suh SP
• Ryang DW

Abstract

BACKGROUND: BACTEC MGIT 960 system(Becton Dickinson, USA; MGIT 960) is a fully automated, noninvasive culture system for mycobacteria, which has been regarded as a sensitive and least labor-intensive method. This study was purposed to evaluate the performance of MGIT 960 compared to BACTEC 460 TB radiometric system(Becton Dickinson, USA; BACTEC 460) and Ogawa media.
METHODS
A total of 1,067 clinical specimens submitted from April to June in 1999 was cultured for acid fast bacilli(AFB). All specimens were digested, decontaminated by the 6% sodium hydroxide(final concentration of 1.5%) and 0.5% N-acetyl-L-cysteine method. All specimens were inoculated into three kinds of media: a MGIT, a BACTEC 12B, and an Ogawa medium. The AFB recovered from cultures were identified to M. tuberculosis complex and MOTT by NAP test.
RESULTS
Of 106 isolates of M. tuberculosis recovered from all culture systems, 101(95.3 %) were detected in the MGIT 960, 95(89.6%) in the BACTEC 460 and 76(71.7%) on Ogawa media. MGIT 960 plus Ogawa media detected 104(98.1%) isolates and BACTEC 460 plus Ogawa media recovered 96(90.6%) isolates. The mean time required for detection of M. tuberculosis was 12.7+/-5.8 days with MGIT 960, 16.2+/-7.7 days with BACTEC 460, and 22.8+/-9.5 days with Ogawa media. The contamination rate were 5.1% for MGIT 960, 2.7% for BACTEC 460, and 6.7% for Ogawa media.
CONCLUSIONS
MGIT 960 is a sensitive and rapid method to isolate M. tuberculosis.

Keyword

Mycobacterium tuberculosis; BACTEC MGIT 960 system(MGIT 960); BACTEC 460 TB radiometric system(BACTEC 460); Ogawa media

MeSH Terms

Acetylcysteine
Mycobacterium tuberculosis*
Mycobacterium*
Sodium
Tuberculosis
Acetylcysteine
Sodium