Exp Mol Med.  1999 Sep;31(3):159-164.

Activation of monocytes, T-lymphocytes and plasma inflammatory markers in angina patients

• Lee WH
• Lee Y
• Kim JR
• Chu JA
• Lee SY
• Jung JO
• Kim JS
• Kim S
• Seo JD
• Rhee SS
• Park JE

Abstract

Inflammation and activation of immune cells have important roles in the pathogenesis of atherosclerosis. We analyzed the plasma levels of inflammatory markers and the degree of activation of peripheral blood monocytes and T-lymphocytes isolated from 12 unstable angina, 12 stable angina, and 12 normal subjects. In 20%-33% of patients, monocytes expressed high basal levels of IL-8, tissue factor, IL-1beta, and monocyte chemoattractant protein-1 mRNA. Furthermore, basal mRNA levels of these cytokines showed strong correlation with each other (p < 0.01 in all combination) but not with tumor necrosis factor-alpha or transforming growth factor-beta1. Plasma level of C-reactive protein was highest in the unstable angina patients (1.63+/-0.70 mg/l) and lowest in the control subjects (0.22+/-0.08 mg/l) (P = 0.03). We also observed a high correlation between C-reactive protein level and the occurrence of minor and major coronary events during 6 months of follow-up. Activation status of T-cells, assessed by the percentage of HLA-DR positive cells, was highest in the unstable angina patients (26.8+/-1.4%) compared with that in the control (14.7+/-1.2%) (P = 0.0053). Our data represent the first case showing that the circulating monocytes in angina patients are activated to a state express numerous proatherogenic cytokines. These results may help to diagnose angina patients according to the inflammatory markers and evaluate the prognosis of the disease.

Keyword

angina; monocyte; T-lymphocytes; inflammation; C-reactive protein

MeSH Terms

Aged
Angina Pectoris/immunology*
Angina Pectoris/diagnosis
Angina, Unstable/immunology*
Angina, Unstable/diagnosis
Biological Markers/blood
C-Reactive Protein/analysis
Cytokines/blood*
Female
HLA-DR Antigens/immunology
Human
Interleukins/blood
Lymphocyte Transformation
Male
Middle Age
Monocyte Chemoattractant Protein-1/blood
Monocytes/metabolism*
RNA, Messenger/metabolism
T-Lymphocytes/metabolism*
Transforming Growth Factor beta/analysis
Tumor Necrosis Factor/analysis