Yonsei Med J.  1997 Jun;38(3):178-186. 10.3349/ymj.1997.38.3.178.

Seroreactivities of proteinases of Candida albicans, C. tropicalis, and C. parapsilosis in sera from various Candida species-infected mice

• Lee KH
• Shin WS
• Park HS
• Park JY
• Koh CM

• KMID: 699947
• DOI: http://doi.org/10.3349/ymj.1997.38.3.178

Abstract

From the culture filtrates of C. albicans, C. tropicalis and C. parapsilosis, proteinases were purified using a series of chromatographic steps consisting of DEAE-Sepharose, Sephacryl S-200 and size-exclusion HPLC which removed contaminating mannoproteins and extraneous proteins. Anti-Candida proteinase antibodies in sera from mice infected with various Candida species were detected using ELISA for serodiagnosis of candidiasis. Three proteinases were blotted by homologous and heterologous anti-proteinase antisera on Western blot analysis. All sera from six Candida species-infected mice were reactive with proteinases of C. albicans, C. tropicalis, and C. parapsilosis, although C. glabrata, C. guilliermondii, and C. krusei did not secrete proteinase. The seroreactivities of proteinase with sera from mice infected with homologous C. albicans and C. tropicalis were higher than those with sera from heterologous Candida species-infected mice. These results suggest that three proteinases have at least one common epitope, but its application for diagnosis of candidiasis should be considered with limits of specificity.

Keyword

Candida proteinase; seroreactivity; HPLC; common epitope

MeSH Terms

Animal
Candida/genetics*
Candida/enzymology*
Candidiasis/enzymology*
Endopeptidases/analysis*
Female
Mice
Mice, Inbred ICR
Species Specificity