Yonsei Med J.  1997 Feb;38(1):52-59. 10.3349/ymj.1997.38.1.52.

Triton X-100 induces apoptosis in human hepatoma cell lines

Affiliations
  • 1Department of Microbiology, Yonsei University College of Medicine, Seoul, Korea.
  • 2Institute for Immunology and Immunological Diseases, Yonsei University College of Medicine, Seoul, Korea.
  • 3Department of Pathology, Yonsei University College of Medicine, Seoul, Korea.
  • 4Department of Internal Medicine, Yonsei University College of Medicine, Seoul, Korea

Abstract

The detergent Triton X-100 was used to establish a model for apoptosis in hepatoma cell lines. The electrophoresis of DNA extracted from 0.01% Triton X-100 treated hepatoma cell lines showed DNA ladder formation, a hallmark of apoptosis. The DNA fragmentation appeared within less than 60 min of the Triton X-100 treatment. Chromatin condensation and apoptotic bodies were observed by hematoxylin and eosin (H & E) stain, and fragmented nucleosome was detected by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labelling (TUNEL) test. Apoptosis was semi-quantitated by measuring the lactate dehydrogenase (LDH) level for cytotoxity. It was found that apoptosis had been induced in more than 90% of the cells treated with Triton X-100 for 150 min. These data show that Triton X-100 efficiently induces the apoptotic cell death in hepatoma cell lines.

Keyword

Triton X-100; apoptosis; hepatoma cells lines; DNA fragmentation; TUNEL test; LDH Introduction

MeSH Terms

Apoptosis*
Carcinoma, Hepatocellular/pathology*
Carcinoma, Hepatocellular/genetics
DNA Fragmentation
Detergents/pharmacology*
Human
Liver Neoplasms/pathology*
Liver Neoplasms/genetics
Octoxynol/pharmacology*
Tumor Cells, Cultured/drug effects
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