Yonsei Med J.  1984 Jun;25(1):39-45. 10.3349/ymj.1984.25.1.39.

Effect of Antioxidants on the Incidence of 7, 12-dimethylbenzanthracene-induced Mammary Tumor in Rats

• Oh SH
• Park YH

• KMID: 692973
• DOI: http://doi.org/10.3349/ymj.1984.25.1.39

Abstract

The inhibitory effect of selenium, vitamin E, and BHA on DMBA-induced mammary tumorigenesis in rats was investigated. Dietary vitamin E (200 IU/Kg diet) alone could not reduce the tumor incidence at 25 weeks after DMBA administration (10mg DMBA/rat) when selenium was deficient. Selenium supplementation (2ppm in drinking water) to rats fed a practical diet (0.17 ppm Se) reduced the tumor incidence to 14.3% from 75% at 27 weeks after DMBA administration. Dietary supplementation of BHA (0.75%) also reduced the incidence of DMBA-induced mammary tumor to 42.9% at 27 weeks after DMBA-treatment. Rats fed a diet deficient in both selenium and vitamin E contained significantly lower glutathione peroxidase activity and higher malondialdehyde in muscle. However, supplementation of selenium or BHA to the rats fed a practical diet did not alter the malondialdehyde content and glutathione peroxidase activities in muscle, skin and mammary gland. Dietary selenium increased the tissue selenium level. DMBA-induced mammary tumorigenesis was reduced by antioxidants tested but the anticarcinogenic effect of selenium or BHA seems to be independent of glutathione peroxi-dase activity.

Keyword

Selenium; BHA; Vitamin E; DMBA; Mammary Tumor

MeSH Terms

9,10-Dimethyl-1,2-benzanthracene
Animal
Antioxidants/pharmacology*
Butylated Hydroxyanisole/pharmacology
Female
Mammary Neoplasms, Experimental/pathology*
Rats
Selenium/pharmacology
Vitamin E/pharmacology