Yonsei Med J.  1977 Jun;18(1):9-18. 10.3349/ymj.1977.18.1.9.

Prednisolone and Glucose-6-phosphatase Activity in Liver Cells

• Shin TS
• Chung IH
• Kim SS

• KMID: 687729
• DOI: http://doi.org/10.3349/ymj.1977.18.1.9

Abstract

Prednisolone, a cortisol analogue, was given intraperitoneally to rats with 5, 10 or 15 doses of 5 mg. per kg. of body weight per day. Sacrificing the animals 24 hours after the last injection, unfixed frozen sections from small pieces of liver tissue were incubated and stained by a modification from the method of Wachstein and Meisel(1965) for glucose-6-phosphatase (G-6-Pase) activity. Some of the tissue blocks were processed for staining with hematoxylin and eosin for histopathological observation. Glucose-6-phosphatase activity, being represented histochemically by brownish-black deposits, was progressively increased after administrations 5 or 10 times. With 15 doses of prednisolone the general histochemical picture of G-6-Pase activity appeared to be similar to that of the control group, except for a different distribution of hepatocytes possessing strong activity. In prednisolone treated rats, the swollen hepatocytes showed a marked, cytoplasmic vacuolization and nuclear pyknosis, particularly in the periportal and midzonal areas of hepatic lobules. Some discussion of the G-6-Pase corticosteroids are presented in terms of their metabolic effects.

MeSH Terms

Animal
Glucose-6-Phosphatase/analysis*
Liver/drug effects
Liver/enzymology*
Male
Prednisolone/pharmacology*
Rats