J Korean Med Sci.  1999 Jun;14(3):245-252. 10.3346/jkms.1999.14.3.245.

Time-dependent expression of ICAM-1 & VCAM-1 on coronaries of the heterotopically transplanted mouse heart

• Lee JR
• Huh JH
• Seo JW
• Suk CJ
• Jeong HM
• Kim EK

• KMID: 646301
• DOI: http://doi.org/10.3346/jkms.1999.14.3.245

Abstract

To investigate the pathogenesis of accelerated graft atherosclerosis after rdiac transplantation, a genetically well-defined and reproducible animal del is required. We performed heterotopic intraabdominal heart transplantation tween the two inbred strains of mice. Forty hearts from B10.A mice were ansplanted into B10.BR mice. Recipients were sacrificed at 1, 3, 5, 7, 14, 28, d 42 days after implantation. The specimens from both donor and recipient were amined with fluorescent immunohistochemistry and the serial histopathologic anges were evaluated. In the donor hearts, ICAM-1 and VCAM-1 expressions were nimal at day 1 and they gradually increased, reaching their peaks on day 5 or and remained unchanged by day 42. However, there were very little expressions the recipients' hearts. Mean percent areas of intima in the donor coronaries vealed progressive increase by day 42. However, those in the recipients cupied consistently less than 5% of the lumen. In conclusion, we demonstrated at a heterotopic murine heart transplantation model was a useful tool to oduce transplantation coronary artery disease and that adhesion molecules on e cardiac allografts were activated very early and remained elevated at all me-points, nonetheless the arterial lesion was detected after day 28 and its ogression was accelerated thereafter.

Keyword

ICAM-1; VCAM-1; Heart transplantation; Coronary atherosclerosis; Graft rejection

MeSH Terms

Animal
Coronary Vessels/pathology
Heart Transplantation*/pathology
Intercellular Adhesion Molecule-1/biosynthesis*
Mice
Myocardium/pathology
Myocardium/metabolism*
Time Factors
Transplantation, Heterotopic*/pathology
Vascular Cell Adhesion Molecule-1/biosynthesis*