Nat Prod Sci.  2025 Mar;31(1):6-12. 10.20307/nps.2025.31.1.6.

Cytotoxicity against MDA-MB-231 Breast Cancer Cells of Fungal Metabolites of Trichoderma sp. Collected from Medicinal Herbal Garden

• Lee D
• Lee JW

• KMID: 2566708
• DOI: http://doi.org/10.20307/nps.2025.31.1.6

Abstract

Trichoderma sp. isolated from the herbal garden is one of the well-known soil fungi, and various metabolites produced by this genus, such as anthraquinones, azaphilones and peptaibols, have been reported to exhibit cytotoxicity against various cancer cells. A large-scale cultivation and a chemical investigation of Trichoderma sp. led to isolation and purification of 10 known compounds from its EtOAc extract. Their structures were elucidated by comparing 1D NMR ( ¹H and ¹³C) and HRESIMS data with previously reported literature. The cytotoxicity of all isolated compounds was measured against MDA-MB-231 breast cancer cells, and koninginin E (10) showed significant inhibitory activity with an IC₅₀ value of 7.3 µM.

Keyword

Soil fungus; Trichoderma sp.; MDA-MB-231; Cytotoxicity; Koninginin E