J Vet Sci.
2025 Jan;26(1):e1. 10.4142/jvs.24225.
Whole genome sequencing analysis of enteropathogenic Escherichia coli from human and companion animals in Korea
- Affiliations
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- 1Division of Biotechnology, Jeonbuk National University, Iksan 54596, Korea
- 2Advanced Institute of Environment and Bioscience, Jeonbuk National University, Iksan 54596, Korea
- 3College of Veterinary Medicine, Chungbuk National University, Cheongju 28644, Korea
- 4Seegene Medical Foundation, Seoul 04805, Korea
- 5Division of Zoonotic and Vector Borne Disease Research, National Institute of Health, Cheongju 28159, Korea
- KMID: 2565091
- DOI: http://doi.org/10.4142/jvs.24225
Abstract
- Importance
This study is essential for comprehending the zoonotic transmission, antimicrobial resistance, and genetic diversity of enteropathogenic Escherichia coli (EPEC).
Objective
To improve our understanding of EPEC, this study focused on analyzing and comparing the genomic characteristics of EPEC isolates from humans and companion animals in Korea.
Methods
The whole genome of 26 EPEC isolates from patients with diarrhea and 20 EPEC isolates from companion animals in Korea were sequenced using the Illumina HiSeq X (Illumina, USA) and Oxford Nanopore MinION (Oxford Nanopore Technologies, UK) platforms.
Results
Most isolates were atypical EPEC, and did not harbor the bfpA gene. The most prevalent virulence genes were found to be ompT (humans: 61.5%; companion animals: 60.0%) followed by lpfA (humans: 46.2%; companion animals: 60.0%). Although pangenome analyses showed no apparent correlation among the origin of the strains, virulence profiles, and antimicrobial resistance profiles, isolates included in clade A obtained from both humans and companion animals exhibited high similarity. Additionally, all the isolates included in clade A encoded the ompT gene and did not encode the hlyE gene. The two isolates from companion animals harbored an incomplete bundle-forming pilus region encoding bfpA and bfpB. Moreover, the type IV secretion system-associated genes tra and trb were found in the bfpA-encoding isolates from humans.
Conclusions and Relevance
Whole-genome sequencing enabled a more accurate analysis of the phylogenetic structure of EPEC and provided better insights into the understanding of EPEC epidemiology and pathogenicity.
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