J Nutr Health.  2024 Dec;57(6):567-579. 10.4163/jnh.2024.57.6.567.

Anti-colitis efficacy of oxyresveratrol isolated from mulberry twig in dextran sulfate sodium-induced mouse colitis

• Cui X
• Lee J
• Choi SW
• Kim E

• KMID: 2564165
• DOI: http://doi.org/10.4163/jnh.2024.57.6.567

Abstract

Purpose
Ulcerative colitis (UC) is a chronic inflammatory bowel disease characterized by inflammation arising in the colonic mucosa. Recently, the incidence of UC has been rapidly increasing due to Westernized lifestyles. If UC persists for a long time (more than 10 years), it is known to elevate the risk of colorectal cancer. In an earlier study, we reported that the mulberry twig (MT) water extract effectively alleviated colitis in mice. In this study, we isolated oxyresveratrol (OXY) from MT as a principal component and compared the anticolitis efficacy of the MT water extract and OXY in a dextran sulfate sodium (DSS)-induced mouse colitis model.
Methods
Six-week-old male ICR mice were divided into four groups: control, DSS, DSS+MT, and DSS+OXY. All mice, except those in the control group, were administered 3% DSS in drinking water for 7 days. During the DSS feeding period, the mice in the DSS+MT and DSS+OXY groups were orally administered MT water extract (5 g/kg body weight [BW]) or OXY (300 mg/kg BW) once daily.
Results
OXY administration significantly suppressed the disease activity index, DSS-induced colonic pathophysiological changes, and the bromodeoxyuridine (BrdU) labeling index of colonic mucosal cells compared to the DSS and DSS+MT groups. The levels of plasma tumor necrosis factor (TNF)-α, interleukin (IL)-6, and nitric oxide (NO), as well as colonic cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) gene expression and myeloperoxidase (MPO) activity, were significantly decreased in the OXY group compared to the DSS group.
Conclusion
These findings suggest that OXY effectively improves mouse colitis by suppressing the colonic inflammatory response and may serve as a potential adjuvant treatment for colitis.

Keyword

colitis; Morus alba; oxyresveratrol; dextran sulfate sodium; mouse