Ann Lab Med.  2025 Jan;45(1):101-104. 10.3343/alm.2024.0182.

Ability of the Capillary Electrophoresis-based HbA1c Assay to Detect Rare Hemoglobin Variants

Affiliations
  • 1Clinical Pathology Unit, Hub Laboratory, AUSL della Romagna, Cesena, Italy
  • 2Laboratory of Human Genetics-IRCCS Istituto Giannina Gaslini, Genoa, Italy
  • 3Sebia Italia srl, Bagno a Ripoli, Firenze, Italy


Figure

  • Fig. 1 Hb analysis using capillary electrophoresis. Examples of normal electropherograms obtains the HbA1c kit (A) and HEMOGLOBIN(E) kit (B). Atypical electropherograms obtained using the HbA1c kit (C, E, G) and normal electropherograms obtained using the HEMOGLOBIN(E) kit (D, F, H) for samples from three patients with Hb Bleuland, Hb La Desirade, and Hb Novara.


Reference

References

1. Dessi M, Pieri M, Pignalosa S, Martino FG, Zenobi R. 2015; Performances of capillary electrophoresis and HPLC methods in HbA1c determination: diagnostic accuracy in HbS and HbD-Iran variants' presence. J Clin Lab Anal. 29:57–60. DOI: 10.1002/jcla.21728. PMID: 24687307. PMCID: PMC6807185.
2. Peeters B, Brandt I, Desmet K, Harteveld CL, Kieffer D. 2016; Hb Melusine and Hb Athens-Georgia: potentially underreported in the Belgian population? Four cases demonstrating the lack of detection using common CE-HPLC methods either for glycated hemoglobin (HbA1c) analysis or Hb variant screening. Acta Clin Belg. 71:458–461. DOI: 10.1080/17843286.2016.1203559. PMID: 27368113.
3. Harteveld CL, Versteegh FGA, Kok PJMJ, van Rooijen-Nijdam IH, van Delft P, Giordano PC. 2006; Hb Bleuland [α108(G15) Thr→Asn, A C C→A A C (α2)]: a new abnormal hemoglobin associated with a mild α-thalassemia phenotype. Hemoglobin. 30:349–54. DOI: 10.1080/03630260600755351. PMID: 16840225.
4. Wajcman H, Traeger-Synodinos J, Papassotiriou I, Giordano PC, Harteveld CL, Baudin-Creuza V, et al. 2008; Unstable and thalassemic alpha chain hemoglobin variants: a cause of Hb H disease and thalassemia intermedia. Hemoglobin. 32:327–49. DOI: 10.1080/03630260802173833. PMID: 18654884.
5. Strickland SW, Campbell ST, Little RR, Bruns DE, Bazydlo LAL. 2018; Recognition of rare hemoglobin variants by hemoglobin A1c measurement procedures. Clin Chim Acta. 476:67–74. DOI: 10.1016/j.cca.2017.11.012. PMID: 29154790.
6. Kamseng P, Trakulsrichai S, Trachoo O, Yimniam W, Panthan B, Jittorntam P, et al. 2017; Low oxygen saturation and severe anemia in compound heterozygous Hb Louisville [β42(CD1) Phe→Leu] and Hb La Desirade [β129(H7)Ala→Val]. Hematology. 22:114–8. DOI: 10.1080/10245332.2016.1231989. PMID: 27670359.
7. Alkindi S, Al Zadjali S, Al Rawahi M, Al Haddabi H, Daar S, ElSadek R A, et al. 2021; Clinical and laboratory features of hemoglobin La Desirade variant in association with sickle cell and alpha thalassemia genes. Mediterr J Hematol Infect Dis. 13:e2021010. DOI: 10.4084/mjhid.2021.010. PMID: 33489049. PMCID: PMC7813281. PMID: 7a3093bc59dd4176ad153ffc2497e438.
8. Crespi I, Campisi M.P, Duranti D, Serino R, Saliva E, Marchiando M, et al. 2018; Incidental detection of a new hemoglobin beta variant performing HbA1c measurement. Biochim Clin. 42:E56–8.
9. Yuan Y, Zhou X, Gao L, Ren Q, Ji L. 2020; Silent hemoglobin variant during capillary electrophoresis: a case report. J Diabetes Investig. 11:1014–7. DOI: 10.1111/jdi.13222. PMID: 32020779. PMCID: PMC7378421. PMID: 73fbd5bdccc04e34aeb20aed34dd999b.
10. G Deschamps F Robert D Simonin . SEBIA. 2015. Dec. 29. Analysis and assay of glycated haemoglobins by capillary electrophoresis, buffer compositions and kits for capillary electrophores. United States patent US 9.222,913B2.
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