Clin Exp Otorhinolaryngol.
2024 Nov;17(4):282-291. 10.21053/ceo.2024.00068.
Video Head Impulse Test Coherence Predicts Vertigo Recovery in Sudden Sensorineural Hearing Loss With Vertigo
- Affiliations
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- 1Department of Biomedical Engineering, College of Engineering, National Cheng Kung University, Tainan, Taiwan
- 2Department of Otorhinolaryngology-Head and Neck Surgery, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan
- 3School of Medicine, National Defense Medical Center, Taipei, Taiwan
- 4School of Medicine, National Sun Yat-sen University, Kaohsiung, Taiwan
- 5Shu-Zen Junior College of Medicine and Management, Kaohsiung, Taiwan
- 6Department of Special Education, College of Education, National Kaohsiung Normal University, Kaohsiung, Taiwan
- 7Medical Device Innovation Center, National Cheng Kung University, Tainan, Taiwan
- 8Institute of Gerontology, College of Medicine, National Cheng Kung University, Tainan, Taiwan
- 9Institute of Medical Informatics, College of Electrical Engineering and Computer Science, National Cheng Kung University, Tainan, Taiwan
- 10Department of Biotechnology and Bioindustry Sciences, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan
- KMID: 2561361
- DOI: http://doi.org/10.21053/ceo.2024.00068
Abstract
Objectives
. Our study aimed to explore the role of the potassium channel KCNK1 in head and neck squamous cell carcinoma, focusing on its impact on tumor growth, invasion, and metastasis. We also investigated the therapeutic potential of quinidine, a known KCNK1 inhibitor, in both in vitro cell lines and a zebrafish patient-derived xenograft (PDX) model.
Methods
. We established primary cell cultures from head and neck cancer tissues and employed the FaDu cell line for in vitro studies, modulating KCNK1 expression through overexpression and knockdown techniques. We evaluated cell migration, invasion, and proliferation. Additionally, we developed a zebrafish PDX model to assess the impact of quinidine on tumor growth and metastasis in vivo. RNA sequencing and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were conducted to elucidate the molecular mechanisms underlying the role of KCNK1 in cancer progression.
Results
. Overexpression of KCNK1 in FaDu cells resulted in enhanced cell migration and invasion, whereas its knockdown diminished these processes. In the zebrafish PDX model, quinidine markedly inhibited tumor growth and metastasis, demonstrating a significant reduction in tumor volume and micrometastasis rates compared to the control groups. The molecular analyses indicated that KCNK1 plays a role in critical signaling pathways associated with tumor growth, such as the Ras and MAPK pathways.
Conclusion
. Our findings highlight the critical role of KCNK1 in promoting tumor growth and metastasis in head and neck cancer. The inhibitory effect of quinidine on tumor progression in the zebrafish PDX model highlights the therapeutic potential of targeting KCNK1. These results suggest that KCNK1 could serve as a valuable therapeutic target for head and neck cancer, warranting further investigation into treatments that target KCNK1.
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