Kosin Med J.  2024 Sep;39(3):195-200. 10.7180/kmj.24.124.

Satellite cell distribution in the medial rectus muscle in cadavers

• Kim CZ
• Lee SJ

• KMID: 2559407
• DOI: http://doi.org/10.7180/kmj.24.124

Abstract

Background
This study aimed to elucidate the potential usefulness of the medial rectus muscle of cadavers for research on satellite cells.
Methods
Twenty-four medial rectus muscles were obtained from 12 cadavers. The control group included six medial rectus muscles from three live adults without brain activity. The muscle fiber diameter and distribution of satellite cells were measured and compared. Immunohistochemistry for myosin heavy chain and the transcription factor PAX7 was performed, and the distributions of myocytes and satellite cells were evaluated.
Results
The average muscle fiber diameter was 142.18±36.49 μm in the cadaver group and 149.34±15.26 μm in the control group, and there was no significant difference between the two groups (p=0.38). The ratio of PAX7(+) cells to the number of muscle fibers was 0.056±0.015 in the control group and 0.006±0.006 in the cadaver group, reflecting a significant difference (p<0.05).
Conclusions
The medial rectus muscles of cadavers can be helpful in studying anatomical morphology; however, their usefulness in muscle satellite cell research appears to be limited.

Keyword

Cadaver; Medial rectus muscles; PAX7; Satellite cell

Figure

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  Fig. 1. (A, B) Light microscope photographs. The medial rectus muscles of the control and cadaver groups showed similar average muscle bundle diameters (hematoxylin and eosin stain, ×200). (C) Muscle bundle diameter distributions are not significantly different between the two groups.

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  Fig. 2. Fluorescence microscopic photographs of the medial rectus muscles of the control (A) and cadaver (B) groups; immunohistochemistry was performed (myosin heavy chain, ×400).

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  Fig. 3. Fluorescence microscopy images of medial rectus muscles with immunohistochemistry of the control (A) and cadaver (B) groups. DAPI, 4′,6-diamidino-2-phenylindole; MyoHC, myosin heavy chain.

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  Fig. 4. DAPI (+) and PAX7 (+) cells were found at the margin of the muscle fiber (A), and the ratio of the PAX7 (+) cells to the number of muscle fibers was higher in the control group than in the cadaver group (p<0.05) (B). DAPI, 4′,6-diamidino-2-phenylindole; MyoHC, myosin heavy chain.

Reference

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