J Periodontal Implant Sci.  2021 Dec;51(6):374-385. 10.5051/jpis.2100700035.

Adjuvant therapy with 1% alendronate gel for experimental periodontitis treatment in rats

• Campos Kajimoto Nd
• Paiva Buischi Yd
• Loomer PM
• Bromage TG
• Ervolino E
• Fucini SE
• Pola NM
• Pirovani BO
• Sismeiro Morábito MJ
• Almeida JMd
• Chaves Furlaneto FA
• Hitomi Nagata MJ

• KMID: 2557516
• DOI: http://doi.org/10.5051/jpis.2100700035

Abstract

Purpose
The aim of this study was to evaluate the effects of locally delivered 1% alendronate (ALN) gel used as an adjunct to non-invasive periodontal therapy.
Methods
Ligature-induced periodontitis was performed in 96 rats. The ligature was tied in the cervical area of the mandibular left first molar. The animals were randomly divided into 4 groups: 1) NT, no treatment; 2) SRP, scaling and root planning; 3) SRP/PLA, SRP followed by filling the periodontal pocket with placebo gel (PLA); and 4) SRP/ALN, SRP followed by filling the periodontal pockets with 1% ALN gel. Histomorphometric (percentage of bone in the furcation region [PBF]) and immunohistochemical (receptor activator of nuclear factor-κB ligand, osteoprotegerin, and tartrate-resistant acid phosphatase) analyses were performed. Data were statistically analyzed, with the threshold of statistical significance set at P≤0.05.
Results
The SRP, SRP/PLA, and SRP/ALN groups presented a higher PBF than the NT group (P≤0.01) at 7, 15, and 30 days. The SRP/ALN group presented a higher PBF than the SRP/PLA group in all experimental periods, as well as a higher PBF than the SRP group at 15 and 30 days. No differences were observed in the immunohistochemical analyses (P>0.05 for all).
Conclusions
Locally delivered 1% ALN gel used as an adjunct to SRP enhanced bone regeneration in the furcation region in a rat model of experimental periodontitis.

Keyword

Alendronate; Dental scaling; Periodontal disease; Periodontitis; Rats