J Periodontal Implant Sci.
2023 Jun;53(3):233-244. 10.5051/jpis.2300100005.
The oral microbiome of implantabutment screw holes compared with the peri-implant sulcus and natural supragingival plaque in healthy individuals
- Affiliations
-
- 1Department of Oral Microbiology, School of Dentistry, Pusan National University, Yangsan, Korea
- 2Oral Genomics Research Center, Pusan National University, Yangsan, Koreav
- 3Interdisciplinary Program of Genomic Science, Pusan National University, Yangsan, Korea
- 4Department of Biomedical Informatics, School of Medicine, Pusan National University, Busan, Korea
- 5Dental Research Institute, BK21 PLUS Project, School of Dentistry, Pusan National University, Yangsan, Korea
- 6Department of Prosthodontics, School of Dentistry, Pusan National University, Yangsan, Korea
- KMID: 2557205
- DOI: http://doi.org/10.5051/jpis.2300100005
Abstract
- Purpose
An implant-supported prosthesis consists of an implant fixture, an abutment, an internal screw that connects the abutment to the implant fixture, and the upper prosthesis. Numerous studies have investigated the microorganisms present on the implant surface, surrounding tissues, and the subgingival microflora associated with peri-implantitis. However, there is limited information regarding the microbiome within the internal screw space. In this study, microbial samples were collected from the supragingival surfaces of natural teeth, the peri-implant sulcus, and the implant-abutment screw hole, in order to characterize the microbiome of the internal screw space in healthy subjects.
Methods
Samples were obtained from the supragingival region of natural teeth, the periimplant sulcus, and the implant screw hole in 20 healthy subjects. DNA was extracted, and the V3–V4 region of the 16S ribosomal RNA was sequenced for microbiome analysis. Alpha diversity, beta diversity, linear discriminant analysis effect size (LEfSe), and network analysis were employed to compare the characteristics of the microbiomes.
Results
We observed significant differences in beta diversity among the samples. Upon analyzing the significant taxa using LEfSe, the microbial composition of the implantabutment screw hole’s microbiome was found to be similar to that of the other sampling sites’ microbiomes. Moreover, the microbiome network analysis revealed a unique network complexity in samples obtained from the implant screw hole compared to those from the other sampling sites.
Conclusions
The bacterial composition of the biofilm collected from the implant-abutment screw hole exhibited significant differences compared to the supra-structure of the implant. Therefore, long-term monitoring and management of not only the peri-implant tissue but also the implant screw are necessary.
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Identification of bacteria from the peri-implant sulcus of orthodontic mini-implants using 16S rDNA clone library
- Comparison of the oral microbial composition between healthy individuals and periodontitis patients in different oral sampling sites using 16S metagenome profiling
- Microbial profiling of peri-implantitis compared to the periodontal microbiota in health and disease using 16S rRNA sequencing
- Efficacy of sonic-powered toothbrushes for plaque removal in patients with peri-implant mucositis
- Risk factors of peri-implantitis: a narrative review
