Korean J Orthod.  2006 Aug;36(4):251-262.

Identification of bacteria from the peri-implant sulcus of orthodontic mini-implants using 16S rDNA clone library

• Lim SH
• Kim KW
• Yoo SY
• Kook JK
• Chang YI

Abstract

OBJECTIVE
The purpose of this study was to compare the bacterial flora at the peri-implant sulcus of the orthodontic mini-implant placed in the alveolar mucosa with the bacterial flora at the adjacent healthy gingival sulcus.
METHODS
Two plaque samples from 7 patients were collected by inserting paper points into the sulcus between the mini-implant and ligature wire connected to the mini-implant head and inflamed alveolar mucosa, and from the gingival sulcus of a healthy tooth adjacent to the mini-implant.
RESULTS
Using 16S rDNA clone library, the 24 kinds of bacteria including Haemophilus aphrophilus, Sphingomonas species, Capnocytophaga species, Prevotella melaninogenica, Lachnospiraceae species, Porphyromonas species, Neisseria flava were identified only from the sulcus around the mini-implant. These bacteria constituted only 9.2% of total clones, and the bacteria identified from both the sulcus around mini-implants and the gingival sulcus constituted 80.4% of total clones. Of these bacteria, clones of Prevotella species, Atopobium rimae, Veillonella species, Streptococcus intermedius/constellatus, Streptococcus salivarius were more frequently isolated from the peri-implant sulcus.
CONCLUSION
This study suggests that a broad epidemiological study is needed to find causative bacteria which induce inflammation from the peri-implant sulcus.

Keyword

Mini-implant; Soft tissue inflammation; Alveolar mucosa; Bacteria

MeSH Terms

Aggregatibacter aphrophilus
Bacteria*
Capnocytophaga
Clone Cells*
DNA, Ribosomal*
Head
Humans
Inflammation
Ligation
Mucous Membrane
Neisseria
Porphyromonas
Prevotella
Prevotella melaninogenica
Sphingomonas
Streptococcus
Tooth
Veillonella
DNA, Ribosomal