J Korean Ophthalmol Soc.  2024 Feb;65(2):139-144. 10.3341/jkos.2024.65.2.139.

Effect of Latanoprostene Bunod on the Permeability of Trabecular Meshwork Cells

• Park HS
• Kim JW

• KMID: 2552432
• DOI: http://doi.org/10.3341/jkos.2024.65.2.139

Abstract

Purpose
To investigate the effects of latanoprostene bunod (LBN) on nitric oxide (NO) production and permeability in human trabecular meshwork cells (HTMC).
Methods
HTMC were treated with 50 and 100 µM LBN and latanoprost free acid (LAT) for 30 minutes. Additionally, 100 µM LBN was co-exposed to 0.5 mM L-NAME (N-Nitroarginine methyl ester). Cellular viability and NO production were measured using MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide) and Griess assays. The permeability and resistance of the HTMC monolayer were evaluated by trans-endothelial electrical resistance (TEER) and carboxyfluorescein permeability.
Results
Exposure to 100 µM LBN led to increased NO production, whereas co-exposure to L-NAME reduced NO production. Treatment with 100 µM LBN decreased the TEER of the HTMC monolayer. LBN exposure heightened carboxyfluorescein permeability, but co-exposure to 100 µM LBN and L-NAME reduced permeability. LAT treatment did not affect NO production or permeability.
Conclusions
LBN increased the permeability of the HTMC monolayer and increased NO production. Therefore, LBN might increase trabecular outflow in addition to promoting uveoscleral outflow.

Keyword

Latanoprost, Latanoprostene bunod, Nitric oxide, Permeability, Trabecular meshwork