Ann Lab Med.  2023 May;43(3):307-309. 10.3343/alm.2023.43.3.307.

Suitability of EDTA-anticoagulated Blood for Natural Killer Cell Activity Testing Using Flow Cytometry

Affiliations
  • 1Department of Laboratory Medicine, Asan Medical Center, Seoul, Korea
  • 2Department of Laboratory Medicine, University of Ulsan College of Medicine, Seoul, Korea


Figure

  • Fig. 1 Comparison of NK cell activity in six paired heparin- and EDTA-anticoagulated samples submitted for flow-cytometric NK cell activity testing. Abbreviation: NK, natural killer.


Reference

1. Henter JI, Horne A, Aricó M, Egeler RM, Filipovich AH, Imashuku S, et al. HLH-2004: Diagnostic and therapeutic guidelines for hemophagocytic lymphohistiocytosis. Pediatr Blood Cancer. 2007; 48:124–31. DOI: 10.1002/pbc.21039. PMID: 16937360.
Article
2. Cichocki F. 2021; Impact of Adaptive NK Cells in Donor Lymphocyte Infusions for the Treatment of Hematological Malignancies. Transplant Cell Ther. 27:101–2. DOI: 10.1016/j.jtct.2021.01.012. PMID: 33781543.
Article
3. Piriou L, Chilmonczyk S, Genetet N, Albina E. 2000; Design of a flow cytometric assay for the determination of natural killer and cytotoxic T-lymphocyte activity in human and in different animal species. Cytometry. 41:289–97. DOI: 10.1002/1097-0320(20001201)41:4<289::AID-CYTO7>3.0.CO;2-5. PMID: 11084614.
Article
4. Vizler C, Nagy T, Kusz E, Glavinas H, Duda E. 2002; Flow cytometric cytotoxicity assay for measuring mammalian and avian NK cell activity. Cytometry. 47:158–62. DOI: 10.1002/cyto.10066. PMID: 11891720.
Article
5. Bromelow KV, Galea-Lauri J, O'Brien ME, Souberbielle BE. 1998; A highly sensitive whole blood natural killer cell assay. J Immunol Methods. 217:177–84. DOI: 10.1016/S0022-1759(98)00109-4. PMID: 9776587.
Article
6. Koh SK, Park J, Kim SE, Lim Y, Phan MT, Kim J, et al. 2022; Natural Killer Cell Expansion and Cytotoxicity Differ Depending on the Culture Medium Used. Ann Lab Med. 42:638–49. DOI: 10.3343/alm.2022.42.6.638. PMID: 35765872. PMCID: PMC9277036.
Article
7. Chung HJ, Park CJ, Lim JH, Jang S, Chi HS, Im HJ, et al. 2010; Establishment of a reference interval for natural killer cell activity through flow cytometry and its clinical application in the diagnosis of hemophagocytic lymphohistiocytosis. Int J Lab Hematol. 32:239–47. DOI: 10.1111/j.1751-553X.2009.01177.x. PMID: 19614711.
Article
8. Son BK, Roberts RL, Ank BJ, Stiehm ER. 1996; Effects of anticoagulant, serum, and temperature on the natural killer activity of human peripheral blood mononuclear cells stored overnight. Clin Diagn Lab Immunol. 3:260–4. DOI: 10.1128/cdli.3.3.260-264.1996. PMID: 8705665. PMCID: PMC170325.
Article
Full Text Links
  • ALM
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr