Ann Lab Med.  2023 Jan;43(1):100-103. 10.3343/alm.2023.43.1.100.

A Case of STIL-TAL1-positive T-lymphoblastic Leukemia With a Minor Philadelphia-positive Clone

• Koo YK
• Kim H
• Shin S

• KMID: 2551610
• DOI: http://doi.org/10.3343/alm.2023.43.1.100

Figure

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  Fig. 1 Morphological and genetic analysis. (A) Blasts on a peripheral blood smear (Wright–Giemsa stain, 1,000×). (B) Agarose gel electrophoresis of the reverse transcription (RT)-PCR product showing the e1a2 (p190) BCR-ABL1 transcript (HemaVision kit; DNA-Diagnostic, Risskov, Denmark). (C) STIL-TAL1 fusion detected using an RNA fusion panel (FusionPlex Pan-Heme Panel; ArcherDX, Boulder, CO, USA). (D) RT-PCR using cDNA and subsequent (E) Sanger sequencing confirmed breakpoints in exons 1 and 3 of STIL and TAL1, respectively. Abbreviations: NTC, non-template control; PC, positive control.

• 

  Fig. 2 Flow cytometry findings of the patient. Abnormal leukemic cells highlighted in red (55.0% of total cells) exhibited a CD2+, cCD3+, CD3+,dim, CD5+, CD7+, CD8+,dim, CD34–, nTdT–, and CD1a– phenotype at diagnosis. Abbreviations: cCD3, cytoplasmic CD3; SS, side scatter.

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