Ann Clin Microbiol.  2023 Dec;26(4):117-124. 10.5145/ACM.2023.26.4.117.

Evaluation of the efficacy of three medical device detergents on bacteria and yeast derived biofilm: a comparative study

• Park K
• Kim MN
• Sung H

• KMID: 2550663
• DOI: http://doi.org/10.5145/ACM.2023.26.4.117

Abstract

Background
This study aimed to evaluate the efficacy of three medical detergents against bacteria and yeast-derived biofilms.
Methods
The biofilm removal efficacy of Empower ^TM (Metrex, USA), Cidezyme ^TM (Johnson and Johnson Medical Inc, USA), and Matrix mint TM (Whiteley Medical, Australia) were compared to that of chlorine bleach. Biofilms were produced using Staphylococcus aureusRN9120, Escherichia coli ATCC35218, Pseudomonas aeruginosa ATCC27853, Candida albicans ATCC14053, and clinical isolates of Enterococcus faecalis, E. coli, Klebsiella pneumoniae, Candida auris, and Trichosporon asahii. The organisms were suspended in tryptic soy broth (TSB) in 96-well microplates and cultured for 72 hours. They were treated with the detergents, and the residual biofilm mass was quantified using crystal violet staining followed by optical density measurements at ₆₂₀ nm (OD ₆₂₀ ).
Results
Empower ^TM and Cidezyme ^TM significantly reduced the biofilm mass derived from all species by > 50% of OD 620 at 37ºC except those from E. faecalis, T. asahii, and C. auris. Matrix mint ^TM had no effect on the biofilms under any condition.
Conclusion
The culture conditions and the species of the biofilm-producing organism influenced the effectiveness of the detergent. Biofilms produced by E. faecalis, C. auris, and T.asahii were resistant to all detergent treatments under all conditions.

Keyword

Biofilm; Detergent; Bacteria; Yeast; Efficacy