Restor Dent Endod.  2022 May;47(2):e18. 10.5395/rde.2022.47.e18.

Effects of calcium silicate cements on neuronal conductivity

• Deniz-Sungur D
• Onur MA
• Akbay E
• Tan G
• Daglı-Comert F
• Sayın TC

• KMID: 2548122
• DOI: http://doi.org/10.5395/rde.2022.47.e18

Abstract

Objectives
This study evaluated alterations in neuronal conductivity related to calcium silicate cements (CSCs) by investigating compound action potentials (cAPs) in rat sciatic nerves.
Materials and Methods
Sciatic nerves were placed in a Tyrode bath and cAPs were recorded before, during, and after the application of test materials for 60-minute control, application, and recovery measurements, respectively. Freshly prepared ProRoot MTA, MTA Angelus, Biodentine, Endosequence RRM-Putty, BioAggregate, and RetroMTA were directly applied onto the nerves. Biopac LabPro version 3.7 was used to record and analyze cAPs. The data were statistically analyzed.
Results
None of the CSCs totally blocked cAPs. RetroMTA, Biodentine, and MTA Angelus caused no significant alteration in cAPs (p > 0.05). Significantly lower cAPs were observed in recovery measurements for BioAggregate than in the control condition (p < 0.05). ProRoot MTA significantly but transiently reduced cAPs in the application period compared to the control period (p < 0.05). Endosequence RRM-Putty significantly reduced cAPs.
Conclusions
Various CSCs may alter cAPs to some extent, but none of the CSCs irreversibly blocked them. The usage of fast-setting CSCs during apexification or regeneration of immature teeth seems safer than slow-setting CSCs due to their more favorable neuronal effects.

Keyword

Apical extrusion; Bioceramics; Calcium silicate cements; Neuronal conductivity; Neurotoxicity

Figure

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  Figure 1 Diagrammatic representation of the experimental set-up. A 3-chambered bath was used, consisting of a stimulating chamber (SC), a perfusion chamber (PC), and a recording chamber (RC). The sciatic nerve is placed through the bath, contacts stimulating electrodes, and passes through the perfusion chamber and reaches the recording electrodes. The Tyrode solution, O2, and CO2 flow in the direction of the arrows and perfuse the nerve.

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  Figure 2 Diagrammatic timetable of the experimental process, showing 3 measurement intervals: baseline, application, and recovery measurements.cAP, compound action potential; CSC, calcium silicate cement.

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  Figure 3 Changes in compound action potential (cAP) amplitudes of the tested calcium silicate cements versus time.

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