Restor Dent Endod.  2021 May;46(2):e17. 10.5395/rde.2021.46.e17.

Hard tissue formation after direct pulp capping with osteostatin and MTA in vivo

• Yoon JH
• Choi SH
• Koh JT
• Lee BN
• Chang HS
• Hwang IN
• Oh WM
• Hwang YC

• KMID: 2548061
• DOI: http://doi.org/10.5395/rde.2021.46.e17

Abstract

Objectives
In recent in vitro study, it was reported that osteostatin (OST) has an odontogenic effect and synergistic effect with mineral trioxide aggregate (MTA) in human dental pulp cells. Therefore, the aim of this study was to evaluate whether OST has a synergistic effect with MTA on hard tissue formation in vivo.
Materials and Methods
Thirty-two maxillary molars of Spraque-Dawley rats were used in this study. An occlusal cavity was prepared and the exposed pulps were randomly divided into 3 groups: group 1 (control; ProRoot MTA), group 2 (OST 100 μM + ProRoot MTA), group 3 (OST 10 mM + ProRoot MTA). Exposed pulps were capped with each material and cavities were restored with resin modified glass ionomer. The animals were sacrificed after 4 weeks. All harvested teeth were scanned with micro-computed tomography (CT). The samples were prepared and hard tissue formation was evaluated histologically. For immunohistochemical analysis, the specimens were sectioned and incubated with primary antibodies against dentin sialoprotein (DSP).
Results
In the micro-CT analysis, it is revealed that OST with ProRoot MTA groups showed more mineralized bridge than the control (p < 0.05). In the H&E staining, it is showed that more quantity of the mineralized dentin bridge was formed in the OST with ProRoot MTA group compared to the control (p < 0.05). In all groups, DSP was expressed in newly formed reparative dentin area.
Conclusions
OST can be a supplementary pulp capping material when used with MTA to make synergistic effect in hard tissue formation.

Keyword

Direct pulp capping; Mineralization, MTA; Osteostatin

Figure

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  Figure 1 Micro-computed tomography images of pulp capped rat molar teeth using mineral trioxide aggregate (MTA) with or without Osteostatin (OST). OST groups (100 μM, 10 mM) showed more hard tissue formation than MTA group. (A) ProRoot MTA, sagittal view (B) OST 100 μM + ProRoot MTA, sagittal view (C) OST 10 mM + ProRoot MTA, sagittal view (D) ProRoot MTA, axial view (E) OST 100 μM + ProRoot MTA, axial view (F) OST 10 mM + ProRoot MTA, axial view. White arrow indicates reparative dentin.

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  Figure 2 The relative ratio of newly formed reparative dentin to pulp cavity in micro-computed tomography results. Area of mineralized tissue and pulp cavity was measured by Image J. Mineral trioxide aggregate (MTA) with osteostatin (OST) group significantly showed more reparative dentin formation than MTA alone group.*p < 0.05.

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  Figure 3 Histological appearance of pulp capped rat molar teeth using MTA with or without OST (×40 magnification). Hematoxylin and eosin-stained sections showed reparative dentinal bridge formation in all groups. Reactionary dentin could be seen around canal orifices in all groups. (A) ProRoot mineral trioxide aggregate (MTA) (B) osteostatin (OST) 100 μM + ProRoot MTA (C) OST 10 mM + ProRoot MTA.P, pulp; RD, reparative dentin.*Capping material.

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  Figure 4 The relative ratio of newly formed reparative dentin to pulp cavity in hematoxylin and eosin histologic results. Area of mineralized tissue and pulp cavity was measured by Image J. Mineral trioxide aggregate (MTA) with osteostatin (OST) group significantly showed more reparative dentin formation than MTA alone group.*p < 0.05.

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  Figure 5 Dentin sialoprotein (DSP) expression of pulp capped rat molar teeth using mineral trioxide aggregate (MTA) with or without osteostatin (OST). All experimental group formed dentinal bridge around exposured pulp and root canal and DSP was expressed in newly formed reparative dentin area.(A) ProRoot MTA (×40) (B) ProRoot MTA (×100) (C) ProRoot MTA, negative control (NC) (D) OST 100 μM + ProRoot MTA (×40) (E) OST 100 μM + ProRoot MTA (×100) (F) OST 100 μM + ProRoot MTA, NC (G) OST 10 mM + ProRoot MTA (×40) (H) OST 10 mM + ProRoot MTA (×100) (I) OST 10 mM + ProRoot MTA.The arrowhead indicates immunolabelling of DSP.

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