Korean J Orthod.  2023 Sep;53(5):336-342. 10.4041/kjod23.129.

Association between Taql polymorphism of vitamin D receptor gene and vertical growth of the mandible: A cross-sectional study

• Telatar BC
• Telatar GY
• Saydam F

• KMID: 2546554
• DOI: http://doi.org/10.4041/kjod23.129

Abstract

Objective
To determine whether the gonial angle on digital panoramic radiographs is associated with vitamin D receptor (VDR) Taql polymorphism.
Methods
Genomic DNA samples were collected from the buccal mucosa of patients aged 26–43 years. TaqMan assay for single nucleotide polymorphism genotyping was used to detect the genotype of Taql polymorphism. The gonial angle was measured bilaterally on panoramic radiography. The normal gonial angle was fixed as 121.8°, and it represented the cutoff value for the high gonial angle (HGA) and low gonial angle (LGA) groups. Various genetic models were analyzed, namely dominant (homozygous [AA] vs. heterozygous [AG] + polymorphic [GG]), recessive (AA + AG vs. GG), and additive (AA + GG vs. AG), using the chi-squared test.
Results
The reliability of the gonial angle measurement was analyzed using a random sample (26%) of the tests, with the intra-examiner correlation showing an intra-class correlation coefficient of 0.99. The frequencies of the AA, AG, and GG genotypes of rs731236 polymorphism were 40.5%, 41.9%, and 17.6% in the HGA group and 21.8%, 51.0%, and 27.2% in the LGA group, respectively (P = 0.042). A statistically significant difference was observed in the allele frequencies between the two groups (P = 0.011). Moreover, a significant correlation was observed in the dominant genetic model.
Conclusions
Taql polymorphism in the VDR gene plays a critical role in the vertical growth of the mandible and decreased gonial angle.

Keyword

Genetics; Growth evaluation; Bone biology

Figure

• 

  Figure 1 Schematic flowchart of the study. VDR, vitamin D receptor.

• 

  Figure 2 Gonial angle measurement. The angle between the tangent to the posterior edge of the ramus mandible and the tangent to the lower edge of the corpus mandible is measured on the right (A) and left (B) sides on panoramic radiography.

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