Genomics Inform.  2023 Jun;21(2):e26. 10.5808/gi.23010.

A bioinformatic approach to identify pathogenic variants for Stevens-Johnson syndrome

Affiliations
  • 1Faculty of Pharmacy, Universitas Ahmad Dahlan, Yogyakarta 55164, Indonesia
  • 2Research Centre for Pharmaceutical Ingredients and Traditional Medicine, National Research and Innovation Agency (BRIN), South Tangerang 15314, Indonesia
  • 3Departement of Pharmacy, University of Muhammadiyah Mataram, Mataram 83127, Indonesia
  • 4Center for Vaccine and Drugs, Research Organization for Health, National Research and Innovation Agency (BRIN), South Tangerang 15314, Indonesia
  • 5Department of Chemistry and Biochemistry, University of California, Los Angeles, CA 90095, USA

Abstract

Stevens-Johnson syndrome (SJS) produces a severe hypersensitivity reaction caused by Herpes simplex virus or mycoplasma infection, vaccination, systemic disease, or other agents. Several studies have investigated the genetic susceptibility involved in SJS. To provide further genetic insights into the pathogenesis of SJS, this study prioritized high-impact, SJS-associated pathogenic variants through integrating bioinformatic and population genetic data. First, we identified SJS-associated single nucleotide polymorphisms from the genome-wide association studies catalog, followed by genome annotation with HaploReg and variant validation with Ensembl. Subsequently, expression quantitative trait locus (eQTL) from GTEx identified human genetic variants with differential gene expression across human tissues. Our results indicate that two variants, namely rs2074494 and rs5010528, which are encoded by the HLA-C (human leukocyte antigen C) gene, were found to be differentially expressed in skin. The allele frequencies for rs2074494 and rs5010528 also appear to significantly differ across continents. We highlight the utility of these population-specific HLA-C genetic variants for genetic association studies, and aid in early prognosis and disease treatment of SJS.

Keyword

bioinformatics; genetic variation; genomic; pathogenic variants; Stevens-Johnson syndrome
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