Korean J Dent Mater.  2023 Mar;50(1):9-16. 10.14815/kjdm.2023.50.1.9.

Anti-inflammatory effects of ethanol extracts of the flower buds of Syzygium aromaticum L. on human gingival fibroblast cells treated with Porphyromonas gingivalis lipopolysaccharide

• Lim YK
• Yoo SY
• Oh HN
• Lee DS
• Kook JK

• KMID: 2541731
• DOI: http://doi.org/10.14815/kjdm.2023.50.1.9

Abstract

This study was conducted to evaluate the cytotoxic and anti-inflammatory effects of the ethanol extract of Syzygium aromaticum L. (clove) buds. The cytotoxicity test was performed by cell counting method using hTERT-hNOF cells, a human immortalized gingival fibroblast cell line. To test the anti-inflammatory effects, the hTERT-hNOF cells were treated with lipopolysaccharide (LPS) extracted from Porphyromonas gingivalis KCOM 2804 (PgLPS) and ethanol extract of clove buds. The expression levels of PGE₂, IL-6, and IL-8 were measured by enzyme-linked immunosorbent assay. The cytotoxicity test data showed a cell viability of ≧ 82% in hTERT-hNOF cells treated with 10 to 80 µg/mL of the ethanol extract of clove buds. The anti-inflammatory test data showed that the expression of PGE₂ by PgLPS treatment was reduced to the level of the negative control group by treatment with 10 µg/mL or more of the ethanol extract of clove buds. In group treated with PgLPS and 40 µg/mL of clove bud ethanol extract, the expression levels of IL-6 and IL-8 in were inhibited by 75% and 77%, respectively (p<0.05), compared to the positive control (PgLPS treatment) group. These results suggest that the ethanol extract of clove buds can be used in developing oral hygine products for preventing periodontal disease.

Keyword

Anti-inflammation; Clove flower buds; Lipopolysaccharide; Porphyromonas gingivalis