Korean J Transplant.  2022 Nov;36(Supple 1):S31. 10.4285/ATW2022.F-1279.

The effect of retrograde venous renal reperfusion on ischemia-reperfusion injury in rabbits

• Rysmakhanov M
• Karakaya E
• Akdur A
• Haberal M

• KMID: 2535203
• DOI: http://doi.org/10.4285/ATW2022.F-1279

Abstract

Background
Ischemia-reperfusion injury (IRI) of a kidney graft is still a current problem in transplantology. The aim of this research was study of the effect of kidney retrograde venous reperfusion (RVR) on IRI.
Methods
The seven New Zealand white male rabbits used. Conditionally, two groups were formed: main group including seven left kidneys of rabbits, which underwent RVR before typical antegrade arterial reperfusion; control group including seven right kidneys, which underwent antegrade arterial reperfusion without RVR. After laparotomy, isolation of the left and right kidneys performed and with isolation vessels and adrenal veins. After 15 minutes of ischemia, the kidneys washed with perfusion solution with evacuation of perfusate through the adrenal veins. Then, a prior retrograde venous reperfusion of the left kidney performed, followed by a typical antegrade arterial reperfusion of both kidneys. After 7 days, euthanasia performed and both kidneys of rabbits were taken for histological examination. Histological changes were evaluated by seven parameters with gradations from 0 to 3, where: 0 no changes; 1 minimal; 2 moderate; 3 severe. Bakent University Ethical Committee for Experimen-tal Research on Animals approved this study.
Results
According histological characteristics of the kidneys, there were no significant differences between the two groups when comparing histological changes in four parameters (Table 1). However, the degree of vacuolization, glomerular shrinkage and nuclear apoptosis in the tissue samples of the group with RVR are less pronounced compared to the control group (P<0.05).
Conclusions
The results of the experimental study showed that the use of RVR could reduce the degree of kidney IRI.