J Dent Rehabil Appl Sci.  2021 Mar;37(1):23-30. 10.14368/jdras.2021.37.1.23.

Investigation of effect of zirconia on osseointegration by surface treatments

• Jeong JW
• Song YG

• KMID: 2525975
• DOI: http://doi.org/10.14368/jdras.2021.37.1.23

Abstract

Purpose
The aim of this study was to investigate effect of zirconia on osseointegration and Surface appearance by surface treatments using various acid solution. Materials and Methods: The prepared zirconia disks were treated with hydrofluoric acid solution and photo-assisted etching under various condition. The surface was analyzed by SEM and the surface roughness was analyzed by using surface profiler. The osteogenic effect of MC3T3-E1 cells was assessed via fluorescent staining observation and reverse transcriptase-polymerase chain reaction (RT-PCR). Results: Various roughness were obtained according to the surface treatment method. The surface roughness increased in the group treated with hydrofluoric acid solution, but that had week network structure. In the method using photo-assisted etching, the surface roughness increased in micro units. Cell reaction showed better results in the photo-assisted etching group than in the hydrofluoric acid-treated group (P < 0.05). And it showed even osteoblastic cell distribution in photo-assisted etching group. Conclusion: As a result, the photo-assisted etching method is more effective than the simple acid solution treatment for zirconia treatment for osseointegration.

Keyword

zirconia; osseointegration; surface treatment

Figure

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  Fig. 1 Schematic diagram of photo-assisted etching.

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  Fig. 2 SEM images with different magnification (A) 1000, (B) 3000, (C) 10000, (D) 30000 times of HNO3 etching group.

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  Fig. 3 SEM images with different magnification (A) 1000, (B) 3000, (C) 10000, (D) 30000 times of HF etching group.

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  Fig. 4 SEM images with different magnification (A) 1000, (B) 3000, (C) 10000, (D) 30000 times of photo-assisted etching group.

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  Fig. 5 Fluorescent staining observation of MC3T3-E1 cells cultured on different surface treated zirconia. A is HNO3 etching group, B is HF etching group, C is photo-assisted etching group, D is no treated control group.

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  Fig. 6 Collagen I, Osterix, Osteocalcin, Runx2 gene expression after 24 hours. Different superscript letters indicate significant difference (P < 0.05) A is HNO3 etching group, B is HF etching group, C is photo-assisted etching group, D is no treated control group.

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  Fig. 7 Comparison of fold change in regulation of 4 genes as assessed by microarray and quantitative RT-PCT with β-actin as an internal control. Different superscript letters indicate significant difference (P < 0.05) A is HNO3 etching group, B is HF etching group, C is photo-assisted etching group, D is no treated control group.

Reference

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