Maxillofac Plast Reconstr Surg.  2021;43(1):5. 10.1186/s40902-021-00290-x.

Comparative study of bone regeneration using fibrin sealant with xenograft in rabbit sinus: pilot study

• Choi WH
• Kim YD
• Song JM
• Shin SH

• KMID: 2525741
• DOI: http://doi.org/10.1186/s40902-021-00290-x

Abstract

Background
Stability of the grafted bone volume is one of the important factors to the success of alveolar bone grafts. For this, platelet-rich plasma (PRP) or fibrin sealant is mixed with the bone graft material. Bio-Oss^® is a protein-free bovine mineral commonly used in bone graft procedures. The grafting particles are commonly combined with a standard fibrin sealant (Tisseel^® ) to fabricate a plastic implantable product. The purpose of this experiment was to evaluate the efficacy of fibrin sealant (Tisseel^® ) in bone regeneration performance in a rabbit maxillary sinus model.
Methods
A total of five 3.5 kg weight New Zealand white rabbits were used for the study. After elevating the sinus membrane in both maxillary sinus cavities, Bio-Oss^® mixed with normal saline (group 1) was filled into the right side, and Tisseel^® mixed Bio-Oss^® (group 2) was inserted into the other side. The bone mineral density and bone volume were analyzed with microscopic computed tomography (micro-CT) and histomorphometric 12 weeks after application.
Results
Histologically, new bone formation rate was 14.8%, and grafted bone rate was 70.5% in group 1. In group 2, they were 18.5% and 60.4%, respectively. According to micro-CT analysis, bone mineral density (mg/cm³ , BMD) was 2.5% larger in group 1.
Conclusions
The findings from this study suggest that, although the difference in the bone formation between group 1 and group 2 appears to be insignificant, group 2 had an advantage in using smaller amount of bone substances to achieve the reliable bone formation.

Keyword

Animal models; Xenograft; Bone graft; Maxillary sinus; Fibrin sealant