Lab Med Qual Assur.  2021 Dec;43(4):214-216. 10.15263/jlmqa.2021.43.4.214.

Comparison Study of Molecular Diagnostic Reagents for COVID-19 Pooling Test

• Seo DH
• Son Gj
• Kang Jw

• KMID: 2524190
• DOI: http://doi.org/10.15263/jlmqa.2021.43.4.214

Abstract

n Korea, real-time reverse transcription-polymerase chain reaction (PCR) is performed on individual or pooled samples to diagnosis coronavirus disease 2019 (COVID-19). New developed LabGun COVID-19 ExoFast RT-PCR Kit (LabGenomics, Korea) was compared to PowerCheck SARS-CoV-2 Real-time PCR Kit (KogeneBiotech, Korea) for its properness about COVID-19 pooling test. LabGun kit showed 98.75% concordance for positive samples and 100% concordance for negative samples tested by PowerCheck kit. Agarose gel electrophoresis study revealed similar PCR products band intensities for two testing kits. In conclusion, LabGun kit could be used for COVID-19 pooling test.

Keyword

COVID-19; RT-PCR testing; Pooling test