Nat Prod Sci.  2021 Sep;27(3):176-182. 10.20307/nps.2021.27.3.176.

Anti-inflammatory and Cytotoxic Activities of Phenolic Compounds from Broussonetia kazinoki

• Vu NK
• Le TT
• Woo MH
• Min BS

• KMID: 2521087
• DOI: http://doi.org/10.20307/nps.2021.27.3.176

Abstract

The phytochemical investigation of Broussonetia kazinoki roots led to the isolation of ten compounds, including six flavonoids (1–6), two lignans (7 and 8), and two coumarins (9 and 10) by comparing their ¹H and ¹³C NMR spectra with reference values. To the best of our knowledge, compounds 9 and 10 were isolated from this plant for the first time. Among the ten isolates, compounds 2, 4, and 6 exhibited inhibitory effects against lipopolysaccharide (LPS)-induced nitric oxide (NO) production in macrophage RAW264.7 cells with IC₅₀ values of 11.98, 10.16, and 24.06 μM, respectively. Furthermore, compounds 2, 4, and 6 reduced LPS-induced inducible nitric oxide synthase (iNOS) expression in a dose-dependent manner. Pre-incubation of cells with these compounds also significantly suppressed LPS-induced COX-2 protein expression. Compounds 2, 4, and 6 also showed cytotoxic activity against HL-60 cells with IC₅₀ values ranging between 46.43 and 94.06 μM.

Keyword

Broussonetia kazinoki; Moraceae; flavonoid; lignan; anti-inflammatory activity; cytotoxic activity