J Korean Assoc Oral Maxillofac Surg.  2021 Aug;47(4):269-278. 10.5125/jkaoms.2021.47.4.269.

Comparison of immunohistochemical analysis on sinus augmentation using demineralized tooth graft and bovine bone

• Sohn DS
• Kim JR
• Kim HG
• Choi HS
• Moon YS

• KMID: 2519824
• DOI: http://doi.org/10.5125/jkaoms.2021.47.4.269

Abstract

Objectives
The purpose of this animal research was to compare bone regeneration in augmented rabbit maxillary sinuses treated with demineralized particulate human-tooth graft and anorganic bovine bone by immunohistochemical analysis.
Materials and Methods
Piezoelectric bilateral sinus augmentation was performed in eight adult rabbits. In the control group, anorganic bovine was grafted in the maxillary sinus following elevation of the sinus membrane. In the experimental group, demineralized human particulate tooth bone was grafted in the sinus. Bone regeneration in augmented sinuses was evaluated by immunohistochemical analysis using various markers of osteoprogenitor cells.
Results
The number of bromodeoxyuridine-labeled cells was significantly higher in the experimental group than in the control group at eight weeks. The immunoreactivity of proliferating-cell nuclear antigen was increased slightly in the experimental group relative to the control group at eight weeks. Other bone markers were expressed equally in the two groups.
Conclusion
In the rabbit maxillary sinus, higher osteoinduction was correlated with demineralized human particulate tooth bone grafting than with anorganic bovine grafting.

Keyword

Maxillary sinus lift; Demineralized tooth bone; Replaceable bony window; Immunohistochemical assay

Figure

• 

  Fig. 1 In the control group, Bio-Oss material was grafted into the new compartments established under the elevated sinus membrane on the left side. In the experimental group, demineralized particulate human tooth bone was grafted into the same area on the right side (A). In both groups, the replaceable bony window was replaced over the bone graft (B). (BO: Bio-Oss, dTB: demineralized particulate human tooth bone, rB: replaceable bone)

• 

  Fig. 2 Coronal sections of a rabbit nasal cavity stained with H&E (×12.5) at two weeks (2W) (A) and eight weeks (8W) (B) after surgery. In each image, the left side shows the grafted sinus treated with Bio-Oss material after maxillary sinus membrane elevation with replacement of the bony window, while the right side shows the grafted sinus treated with demineralized particulate human tooth bone with replacement of the bony window. (BO: Bio-Oss, dTB: demineralized particulate human tooth bone, NS: nasal septum)

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  Fig. 3 Immunohistochemical staining (×200) for bromodeoxyuridine (BrdU) in the control group at two weeks (A) and eight weeks (B) and the experimental group at two weeks (C) and eight weeks (D). BrdU-positive cells (arrows) confirmed the existence of osteoblasts on the surface of newly formed bone and graft materials. (BO: Bio-Oss, N: newly formed bone, dTB: demineralized particulate human tooth bone)

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  Fig. 4 Histomorphometric measurements of the number of bromodeoxyuridine (BrdU)-labeled cells per 1 mm2 of soft tissue of the augmented sinus. The soft tissue area contains fibrous tissue, vascular tissue, and bone marrow of the augmented sinus (*P<0.05). (NS: not significant)

• 

  Fig. 5 Immunohistochemical staining (×200) with proliferating-cell nuclear antigen (PCNA) in the control group at two weeks (A) and eight weeks (B) and the experimental group at two weeks (C) and eight weeks (D). Arrows indicate PCNA-positive cells. (BO: Bio-Oss, N: newly formed bone, dTB: demineralized particulate human tooth bone)

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  Fig. 6 Immunohistochemical staining (×100) for type I collagen in the control group at two weeks (A) and eight weeks (B) and the experimental group at two weeks (C) and eight weeks (D). (BO: Bio-Oss, N: newly formed bone, dTB: demineralized particulate human tooth bone)

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  Fig. 7 Immunohistochemical staining (×100) for osteopontin in the control group at two weeks (A) and eight weeks (B) and the experimental group at two weeks (C) and eight weeks (D). (BO: Bio-Oss, N: newly formed bone, dTB: demineralized particulate human tooth bone)

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  Fig. 8 Immunohistochemical staining (×100) for osteocalcin in the control group at two weeks (A) and eight weeks (B) and the experimental group at two weeks (C) and eight weeks (D). (BO: Bio-Oss, N: newly formed bone, dTB: demineralized particulate human tooth bone)

Reference

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