Korean J Parasitol.
2021 Feb;59(1):83-88. 10.3347/kjp.2021.59.1.83.
Parvatrema duboisi (Digenea: Gymnophallidae) Life Cycle Stages in Manila Clams, Ruditapes philippinarum, from Aphae-do (Island), Shinan-gun, Korea
- Jung BK
1 - Chang T1
- Shin H1
- Ryoo S1
- Hong S1
- Lee J1
- Song H1
- Cho J1
- Kim DG1
- Jun H2
- Kim MJ3
- Won EJ4
- Han ET5
- Shin EH6,7
- Chai JY1,6
- Affiliations
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- 1Institute of Parasitic Diseases, Korea Association of Health Promotion, Seoul 07649, Korea
- 2Department of Tropical Medicine, Inha University College of Medicine, Incheon 22212, Korea
- 3Department of Infectious Diseases, Asan Medical Center, Seoul 05505, Korea
- 4Department of Parasitology and Tropical Medicine, Chonnam National University Medical School, Gwangju 61469, Korea
- 5Department of Medical Environmental Biology and Tropical Medicine, Kangwon National University, Chuncheon 24341, Korea
- 6Department of Tropical Medicine and Parasitology, Seoul National University College of Medicine, Seoul 03080, Korea
- 7Seoul National University Bundang Hospital, Seongnam 13620, Korea
- KMID: 2512990
- DOI: http://doi.org/10.3347/kjp.2021.59.1.83
Abstract
- Life cycle stages, including daughter sporocysts, cercariae, and metacercariae, of Parvatrema duboisi (Dollfus, 1923) Bartoli, 1974 (Digenea: Gymnophallidae) have been found in the Manila clam Ruditapes philippinarum from Aphaedo (Island), Shinan-gun, Jeollanam-do, Korea. The daughter sporocysts were elongated sac-like and 307-570 (av. 395) μm long and 101-213 (av. 157) μm wide. Most of the daughter sporocysts contained 15-20 furcocercous cercariae each. The cercariae measured 112-146 (av. 134) μm in total length and 35-46 (av. 40) μm in width, with 69-92 (av. 85) μm long body and 39-54 (av. 49) μm long tail. The metacercariae were 210-250 (av. 231) μm in length and 170-195 (av. 185) μm in width, and characterized by having a large oral sucker, genital pore some distance anterior to the ventral sucker, no ventral pit, and 1 compact or slightly lobed vitellarium, strongly suggesting P. duboisi. The metacercariae were experimentally infected to ICR mice, and adults were recovered at day 7 post-infection. The adult flukes were morphologically similar to the metacercariae except in the presence of up to 20 eggs in the uterus. The daughter sporocysts and metacercariae were molecularly (ITS1-5.8S rDNA-ITS2) analyzed to confirm the species, and the results showed 99.8-99.9% identity with P. duboisi reported from Kyushu, Japan and Gochang, Korea. These results confirmed the presence of various life cycle stages of P. duboisi in the Manila clam, R. philippinarum, playing the role of the first as well as the second intermediate host, on Aphae-do (Island), Shinan-gun, Korea.
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