Ann Lab Med.  2021 May;41(3):328-332. 10.3343/alm.2021.41.3.328.

Promyelocytic Blast Phase of Chronic Myeloid Leukemia, BCR-ABL1-Positive: Points to be Considered at Diagnosis

• Kim B
• Chi HY
• Yoon YA
• Choi YJ

• KMID: 2512692
• DOI: http://doi.org/10.3343/alm.2021.41.3.328

Figure

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  Fig. 1 Results of BM study, RT-qPCR, and FISH assay at the diagnosis of blast phase of CML. (A) Abnormal promyelocytes on a BM aspirate smear (Wright–Giemsa stain, × 1,000). (B) RT-qPCR analysis of BCR-ABL1 translocation (HemaVision-28N, DNA Diagnostic, Risskov, Denmark), showing a 397-bp band in the M6B split-out PCR and a 353-bp band in the M8C split-out PCR, indicating BCR-ABL1 (b3a2) and PML-RARA (bcr1 isoform) fusion transcripts. (C and D) FISH using a break-apart probe for BCR-ABL1 and PML-RARA fusion genes (Cytocell, Cytocell Ltd, Oxford Gene Technology, Cambridge, UK) showed two fusion signals, one green and one red, suggesting both BCRABL1 and PML-RARA rearrangements. Abbreviations: BM, bone marrow; CML, chronic myeloid leukemia; FISH, fluorescence in-situ hybridization; RT-qPCR, quantitative reverse-transcription PCR.

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