Tissue Eng Regen Med.
2021 Feb;18(1):143-154. 10.1007/s13770-020-00312-1.
Human Dental Pulp Stem Cells (DPSCs) Therapy in Rescuing Photoreceptors and Establishing a Sodium Iodate-Induced Retinal Degeneration Rat Model
- Lam C1
- Alsaeedi HA2
- Koh AEH3
- Harun MHN1
- Hwei ANM4
- Mok PL3,5,6
- Luu CD7,8
- Yong TK9
- Subbiah SK2,6,10
- Bastion MLC
1
- Affiliations
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- 1Faculty of Medicine, Department of Ophthalmology, UKM Medical Centre, 56000 Cheras, Kuala Lumpur, Malaysia
- 2Department of Medical Microbiology and Parasitology, Faculty of Medicine & Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
- 3Department of Biomedical Science, Faculty of Medicine & Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
- 4Tissue Engineering Centre, UKM Medical Centre, 56000 Cheras, Kuala Lumpur, Malaysia
- 5Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakaka, P.O. Box 2014, Aljouf Province, Saudi Arabia
- 6Genetics and Regenerative Medicine Research Centre, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
- 7Centre for Eye Research Australia, Royal Victorian Eye & Ear Hospital, Melbourne 3002, Australia
- 8Department of Surgery (Ophthalmology), The University of Melbourne, Melbourne 3010, Australia
- 9Brighton Healthcare Suite G-2, Ground Floor, Bio X Centre, Persiaran Cyberpoint Selatan, Cyber 8, 63000 Cyberjaya, Malaysia
- 10Department of Biotechnology, Bharath Institute of Higher Education and Research (BIHER), Chennai, Tamil Nadu, India
- KMID: 2512646
- DOI: http://doi.org/10.1007/s13770-020-00312-1
Abstract
- BACKGROUND
Different methods have been used to inject stem cells into the eye for research. We previously explored the intravitreal route. Here, we investigate the efficacy of intravenous and subretinal-transplanted human dental pulp stem cells (DPSCs) in rescuing the photoreceptors of a sodium iodate-induced retinal degeneration model.
METHODS
Three groups of Sprague Dawley rats were used: intervention, vehicle group and negative control groups (n = 6 in each). Intravenous injection of 60 mg/kg sodium iodate (day 0) induced retinal degeneration. On day 4 postinjection of sodium iodate, the rats in the intervention group received intravenous DPSC and subretinal DPSC in the right eye; rats in the vehicle group received subretinal Hank’s balance salt solution and intravenous normal saline; while negative control group received nothing. Electroretinogram (ERG) was performed to assess the retinal function at day 0 (baseline), day 4, day 11, day 18, day 26, and day 32. By the end of the study at day 32, the rats were euthanized, and both their enucleated eyes were sent for histology.
RESULTS
No significant difference in maximal ERG a-wave (p = 0.107) and b-wave, (p= 0.153) amplitude was seen amongst the experimental groups. However, photopic 30 Hz flicker amplitude of the study eye showed significant differences in the 3 groups (p = 0.032). Within the intervention group, there was an improvement in 30 Hz flicker ERG response of all 6 treated right eyes, which was injected with subretinal DPSC; while the 30 Hz flicker ERG of the nontreated left eyes remained flat. Histology showed improved outer nuclear layer thickness in intervention group; however, findings were not significant compared to the negative and vehicle groups.
CONCLUSION
Combination of subretinal and intravenous injection of DPSCs may have potential to rescue cone function from a NaIO3 -induced retinal injury model.
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