Tissue Eng Regen Med.  2020 Dec;17(6):901-908. 10.1007/s13770-020-00298-w.

Articular Cartilage Regeneration Utilizing Decellularized Human Placental Scaffold, Mesenchymal Stem Cells and Platelet Rich Plasma

• Özdemir E
• Emet A
• Hashemihesar R
• Sinan Yürüker AC
• Kılıc E
• Çetinkaya DU
• Turhan E

• KMID: 2509433
• DOI: http://doi.org/10.1007/s13770-020-00298-w

Abstract

BACKGROUND
Articular cartilage repair has been a challenge in orthopedic practice due to the limited self-regenerative capability. Optimal treatment method for cartilage defects has not been defined. We investigated the effect of decellularized human placental (DHP) scaffold, mesenchymal stem cells (MSC) and platelet-rich plasma (PRP) on hyaline cartilage regeneration in a rat model.
METHODS
An osteochondral defect was created in trochlea region of the femur in all groups, bilaterally. No additional procedure was performed in control group (n = 14). Only the DHP scaffold was applied to the P group (n = 14). The DHP scaffold and 1 x 10⁶ MSCs were applied to the PS group (n = 14). The DHP scaffold and PRP were applied to the PP group (n= 14). The DHP scaffold, 1 x 10⁶ MSCs and PRP were applied to the PSP group (n = 14). Outcome measures at 12 weeks included Pineda histology score and qualitative histology.
Results
The mean Pineda scores of P, PS, PP, and PSP groups were significantly better than the control group (p = 0.031, p = 0.002, p < 0.001, p < 0001, respectively). There was no statistically difference in mean Pineda scores of P, PS, PP, and PSP groups (p > 0.05).
Conclusion
In conclusion, the DHP scaffold appears to be a promising scaffold on hyaline cartilage regeneration. The augmentation of DHP scaffold with MSCs and PRP combinations did not enhance its efficacy on articular cartilage regeneration.

Keyword

Cartilage; Placenta decellularization; Platelet-rich plasma; Mesenchymal stem cells