Ann Lab Med.  2020 Nov;40(6):490-492. 10.3343/alm.2020.40.6.490.

Quality of Ribonucleic Acid Extraction for Real-Time Reverse Transcription-PCR (rRT-PCR) of SARS-CoV-2: Importance of Internal Control Monitoring

• Lee YJ
• Lim Y
• Hur KW
• Sung H
• Kim MN

• KMID: 2507796
• DOI: http://doi.org/10.3343/alm.2020.40.6.490

Figure

• 

  Fig. 1 Trends of Ct values of the IC in rRT-PCR for SARS-CoV-2 detection. At the end of 26th February 2020, in 31 samples, RNA was extracted using the Real-Prep (BioSewoom, Seoul, Korea) system in use, 2–26 (in use), and in 35 samples, RNA was extracted using the new Real-Prep system, 2–26 (new); all of these were submitted to a single run of PCR for comparison. Abbreviations: Ct, cycle threshold; IC, internal control; rRT-PCR, real-time reverse transcription PCR; SARS-CoV-2, severe acute respiratory syndrome coronavirus 2; NP, nasopharyngeal.

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