J Korean Assoc Oral Maxillofac Surg.  2020 Jun;46(3):197-203. 10.5125/jkaoms.2020.46.3.197.

FK506 immunosuppression for submandibular salivary gland allotransplantation in rabbit

• Almansoori AA
• Khentii N
• Ju KW
• Kim B
• Kim SM
• Lee JH

• KMID: 2504306
• DOI: http://doi.org/10.5125/jkaoms.2020.46.3.197

Abstract

Objectives
We compared the outcomes of two different doses of FK506 (tacrolimus) for immunosuppression in submandibular salivary gland (SMG) allotransplantation.
Materials and Methods
Three SMG allotransplantation groups were established (n=6 per group) as follows: allograft rejection control (Allo-Ctrl), low dose (0.08 mg/kg) of FK506 (FK506-L), and high dose (0.16 mg/kg) of FK506 (FK506-H). Allograft survival and rejection were assessed by clinical observation, interleukin-2 levels as determined by enzyme-linked immunosorbent assay, blood sampling for complete blood count (CBC), and histological evaluation.
Results
Body weight and anorexia were higher in the FK506-H group but without a significant difference compared with the FK506-L population. CBC revealed a non-significantly reduced number of changes in the FK506-L group. Four glands in the FK506-H group and two glands in the FK506- L group were viable and functioning post-transplantation.
Conclusion
The survival rate of allotransplanted glands was higher in conjunction with the high dose of 0.16 mg/kg of FK506, with no major difference in the side-effect profile when compared with the low dose of 0.08 mg/kg short-term outcomes.

Keyword

Submandibular salivary gland; Allotransplantation; Immunosuppression; FK506; Tacrolimus

Figure

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  Fig. 1 Intraoperative clinical photographs for submandibular salivary gland (SMG) allotransplantation. A. Complete separation of the SMG in the donor rabbit with an intact linguofacial vein, common carotid artery, and silicon tube connected to Wharton’s duct. B. Harvested SMG over the receiver chin with proper orientation of the vessels. C. After vascular anastomosis and connection to the remaining distal Wharton’s duct of the receiver. D. After skin suturing and drain placement.

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  Fig. 2 Photomicrographs of allotransplanted glands as shown by H&E staining. A. Histological features of an allotransplanted gland with nearly normal glandular parenchyma structure (magnification, ×1.25; scale bar=200 μm). B. The same allotransplanted gland (magnification, ×20; scale bar=200 μm). C. Histological features of an allotransplanted gland with partial loss of the parenchyma (magnification, ×1.25; scale bar=200 μm). D. The same allotransplanted gland (magnification, ×20; scale bar=200 μm). E. Histological features of an allotransplanted gland with loss of the majority of the parenchyma and an abundance of fibrous tissue (magnification, ×1.25; scale bar=800 μm). F. The same allotransplanted gland (magnification, ×20; scale bar=50 μm). G. Histological features of a rejected allotransplanted gland with acini degeneration and marked inflammatory cells infiltration (magnification, ×1.25; scale bar=800 μm). H. The same rejected gland (magnification, ×20; scale bar=50 μm).

Reference

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