J Bacteriol Virol.  2020 Mar;50(1):25-34. 10.4167/jbv.2020.50.1.025.

Expression and Purification of Recombinant Mayaro Virus Envelope Glycoproteins E1 and E2 to Develop a Mayaro Virus Detection System

Affiliations
  • 1BioNano Health Guard Research Center, 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea. marsp@kribb.re.kr, ybshin@kribb.re.kr
  • 2Department of Biochemistry, College of Natural Sciences, Chungnam National University, 99 Daehak-ro, Yuseong-gu, Daejeon 34134, Republic of Korea.
  • 3Department of Clinical Laboratory Science, Daejeon Health Institute of Technology, Daejeon 34504, Republic of Korea.
  • 4Department of General Education, Daejeon Health Institute of Technology, Daejeon 34504, Republic of Korea.
  • 5Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.

Abstract

Mayaro virus (MAYV) is a mosquito-transmitted alphavirus that produces an acute, usually non-fatal, febrile illness including Mayaro fever. Like other alphaviruses, the MAYV E1 and E2 envelope glycoproteins are major viral surface antigens that play a key role in host recognition and infection. Here, we report expression and purification methods for recombinant MAYV E1 (rE1) and rE2 using a baculovirus system. Enzyme-linked immunosorbent assays (ELISA) revealed that rE1 and rE2 were antigenic and reacted with human anti-MAYV IgG and IgM. Cross-reactivity was also confirmed with human anti-Chikungunya virus (CHIKV) IgG and IgM. Furthermore, we developed an immunochromatographic strip test (IST) with rE2 to diagnose MAYV infection. Thus, purified rE2 may be valuable tool for rapidly diagnosing MAYV infection.

Keyword

Mayaro virus (MAYV); immunochromatographic strip test (IST); recombinant envelope glycoprotein 2 (rE2); recombinant envelope glycoprotein 1 (rE1); Baculovirus/insect cell system

MeSH Terms

Alphavirus
Antigens, Surface
Baculoviridae
Enzyme-Linked Immunosorbent Assay
Fever
Glycoproteins*
Humans
Immunoglobulin G
Immunoglobulin M
Antigens, Surface
Glycoproteins
Immunoglobulin G
Immunoglobulin M
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