J Biomed Transl Res.  2019 Dec;20(4):91-98. 10.12729/jbtr.2019.20.4.091.

Effect of dyslipidemia on vascular smooth muscle cell proliferation in a porcine coronary restenosis model

Affiliations
  • 1Futuristic Animal Resource and Research Center & National Primate Research Center, Ochang 28116, Korea. dvmlim96@kribb.re.kr
  • 2Department of Internal Medicine and Cardiovascular Center, Chosun University Hospital, Gwangju 61453, Korea.
  • 3CGBio Co. Ltd, Jangseong, 57248, Korea.
  • 4Cardiovascular Research Center, Chonnam National University Hospital, Gwangju 61469, Korea. mhjeong@chonnam.ac.kr
  • 5Korea Cardiovascular Stent Research Institute, Jangseong 57248, Korea.
  • 6Research Institute of Medical Sciences, Chonnam National University, Gwangju 61186, Korea.
  • 7Departments of Thoracic and Cardiovascular Surgery and Cardiology, Wonkwang University School of Medicin, Iksan 54538, Korea.

Abstract

This study aimed to examine the effect of a mild elevation in serum cholesterol level in a porcine coronary overstretch restenosis model using a balloon angioplasty catheter or drug-eluting coronary stent. Pigs were divided into two groups and were fed a commercial normal diet (CND, n = 4) or a high-fat diet (HFD, n = 4) for 5 weeks. Coronary overstretch injury by balloon angioplasty or stent implantation was induced in the left anterior descending and left circumflex artery after 1 week of feeding. Histopathological analysis was performed at 4 weeks after coronary injury. During the experiment, the total cholesterol level in the HFD group increased by approximately 44.9% (from 65.9 ± 3.21 mg/dL at baseline to 95.5 ± 9.94 mg/dL at 5 weeks). The lumen area in the CND group was reduced in comparison with that in the HFD group after balloon angioplasty. After stent implantation, the injury score showed no significant difference. There were significant differences in the neointimal area (2.7 ± 0.33 mm² in the CND group vs. 3.3 ± 0.34 mm² in the HFD group, p<0.05), lumen area (2.6 ± 0.54 mm² in the CND group vs. 2.0 ± 0.33 mm² in the HFD group, p<0.05), and percent area stenosis (52.0 ± 7.96% in the CND group vs. 62.4 ± 5.15% in the HFD group, p<0.05). Body weight change was not different between the two groups. Increased serum cholesterol level activated vascular smooth muscle cell proliferation in the porcine coronary overstretch model.

Keyword

cholesterol; balloon angioplasty; coronary stents; percutaneous coronary intervention; restenosis

MeSH Terms

Angioplasty, Balloon
Arteries
Body Weight Changes
Catheters
Cell Proliferation*
Cholesterol
Constriction, Pathologic
Coronary Restenosis*
Diet
Diet, High-Fat
Dyslipidemias*
Muscle, Smooth, Vascular*
Percutaneous Coronary Intervention
Stents
Swine
Cholesterol
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