Mycobiology.  2019 Dec;47(4):512-520. 10.1080/12298093.2019.1661566.

Optimization of Growth Medium and Fermentation Conditions for the Production of Laccase3 from Cryphonectria parasitica Using Recombinant Saccharomyces cerevisiae

• Jeong YS
• So KK
• Lee JH
• Kim JM
• Chun GT
• Chun J
• Kim DH

• KMID: 2470184
• DOI: http://doi.org/10.1080/12298093.2019.1661566

Abstract

Statistical experimental methods were used to optimize the medium for mass production of a novel laccase3 (Lac3) by recombinant Saccharomyces cerevisiae TYEGLAC3-1. The basic medium was composed of glucose, casamino acids, yeast nitrogen base without amino acids (YNB w/o AA), tryptophan, and adenine. A one-factor-at-a-time approach followed by the fractional factorial design identified galactose, glutamic acid, and ammonium sulfate, as significant carbon, nitrogen, and mineral sources, respectively. The steepest ascent method and response surface methodology (RSM) determined that the optimal medium was (g/L): galactose, 19.16; glutamic acid, 5.0; and YNB w/o AA, 10.46. In this medium, the Lac3 activity (277.04 mU/mL) was 13.5 times higher than that of the basic medium (20.50 mU/mL). The effect of temperature, pH, agitation (rpm), and aeration (vvm) was further examined in a batch fermenter. The best Lac3 activity was 1176.04 mU/mL at 25 °C, pH 3.5, 100 rpm, and 1 vvm in batch culture.

Keyword

Saccharomyces cerevisiae; Cryphonectria parasitica; fermentation; laccase3; response surface methodology

MeSH Terms

Adenine
Amino Acids
Ammonium Sulfate
Batch Cell Culture Techniques
Carbon
Dihydroergotamine
Fermentation*
Galactose
Glucose
Glutamic Acid
Hydrogen-Ion Concentration
Methods
Miners
Nitrogen
Saccharomyces cerevisiae*
Saccharomyces*
Tryptophan
Yeasts
Adenine
Amino Acids
Ammonium Sulfate
Carbon
Dihydroergotamine
Galactose
Glucose
Glutamic Acid
Nitrogen
Tryptophan